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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

Fig. 3

Effect of SJM on RAW 264.7 phagocytic capability and in vitro anti-inflammation. a The Cayman phagocytosis assay kit was applied to treat the cells with SJM for 8 h. Observing the cells under a microscope revealed that compared with the vehicle group, the SJM-treated cells exhibited enhanced ability in engulfing the fluorescent beads. b The neutral red experiment was conducted to observe the effect of SJM on the phagocytic capability of RAW 264.7. The statistical results showed that the absorbance values of the RAW 264.7 SJM- and LPS-treated cells were significantly higher than that of the control group, indicating that SJM enhances the phagocytic ability of macrophages. cThe RAW 264.7 cells were treated with LPS and SJM for 16 h, and then western blotting was applied to detect the expression of iNOS in these cells. The results revealed that LSP induced RAW 264.7 to synthesize inflammation factor iNOS. However, SJM significantly inhibited LPS from inducing the synthesis of iNOS proteins. # denotes p < 0.05 compared with the control group and * denotes p < 0.05 compared with the LPS group; scale = 100 μm

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