Pro-apoptotic and cytotoxic effects of enriched fraction of Elytranthe parasitica (L.) Danser against HepG2 Hepatocellular carcinoma

Table 1 Effect of EP extract/fractions on % cell viability of HepG2 carcinoma cell line by SRB assay

Concentration (μg/mL)	EP.M	EP.PE	EP.DEE	EP.EA	EP.But	EP.Aq	Gallic acid
0	100.00 ± 0.33	100.00 ± 0.33	100.00 ± 0.33	100.00 ± 0.33	100.30 ± 0.33	100.30 ± 0.30	100.30 ± 0.30
25	96.80 ± 2.40	99.20 ± 5.60	59.30 ± 6.70	80.00 ± 0.50	99.60 ± 5.50	99.80 ± 4.50	53.80 ± 5.66
50	72.30 ± 5.50	99.00 ± 2.10	53.70 ± 11.90	63.10 ± 1.22	99.30 ± 3.80	99.10 ± 2.30	42.90 ± 9.20
100	69.00 ± 3.70	98.40 ± 0.40	40.90 ± 5.60	50.20 ± 4.40	94.80 ± 3.10	98.30 ± 1.50	33.50 ± 4.90
200	42.50 ± 2.10	86.00 ± 9.10	36.60 ± 3.98	38.90 ± 6.20	91.60 ± 7.80	93.00 ± 4.10	24.90 ± 6.89
400	16.40 ± 7.20	83.00 ± 9.00	20.90 ± 7.10	22.90 ± 3.10	72.40 ± 5.40	86.00 ± 3.20	20.70 ± 4.62
IC50	154.10 ± 2.60^c	> 200	56.70 ± 7.80^a	101.30 ± 5.3^b	> 200	> 200	31.00 ± 6.21^a

Results are expressed as mean ± SEM (of three observations) and analyzed using one-way ANOVA followed by Tukey’s post hoc test of significance [where different alphabets ^a-cdenote significant difference (p <0.05)]. EP.M Methanol extract, EP.PE Petroleum ether fraction, EP.DEE Diethyl ether fraction, EP.EA Ethyl Acetate fraction, EP.But Butanol fraction, EP.Aq Aqueous fraction

ISSN: 2662-7671