Fig. 5

Effects of GJT on activation of NF-κB in LPS-stimulated RAW264.7 macrophages. a Cells were pretreated with GJT (0, 62.5, or 125 μg/mL) for 2 h and the treated with LPS (1 μg/mL) for 1 h. Nuclear extracts were prepared and subjected to immunoblotting for NF-κB p65. b Cells were pretreated with or without GJT (125 μg/mL) for 2 h and the treated with LPS (1 μg/mL) for 1 h. The cells were fixed in 4 % (v/v) methanol free formaldehyde solution (pH 7.4), stained with anti-NF-κB p65 (Texas Red). The immunostained cells were then mounted with medium containing DAPI and visualized under an Olympus FLUOVIEW FV10i confocal microscopy. c; untreated control