Fig. 2

Effect of Allium sativum extract on human PBMCs proliferation. Panels from a to i show an example of data generated by CFSE and anti-CD4 stained PBMCs. Panels from j to r show an example of data generated by CFSE and anti-CD8 stained PBMCs. Cells were cultured in different conditions of stimulation and treated with two doses of Allium sativum (A.S.) extract for 4 days. a and j untreated PBMCs b and k, c and l PBMCs treated with A.S. extract at 1 and 2 μg/ml respectively. d and m PBMCs stimulated with PHA. e and n, f and o cells were stimulated with PHA and treated with A.S. extract at 0.5 and 1 μg/ml respectively. g and p cells were stimulated with Okt-3 mAb at 0.1 μg/ml. h and q, i and r PBMCs stimulated with Okt-3 mAb and treated with A.S. extract at 1 and 2 μg/ml respectively. Each peak represents a cycle of cell division. The curves generated by the CFSE profile were analyzed using the proliferation platform of the FlowJo software. Data shown represent results of 4 independent experiments. IP indicates index of proliferation calculated with FlowJo software