Fig. 2

Protective effect of 3-O-methyl quercetin and kaempferol on H₂O₂ induced mitochondria and plasma membrane damage in L132 and L02 cells. a Cells were pretreated with 3-O-methyl quercetin and kaempferol followed by H₂O₂ for 24 h. Cell incubated with 2′,7′-DCFH-DA for 30 min and images were taken using a fluorescence microscope. The intensity of the fluorescence was measured at an excitation wavelength of 485 nm and an emission wavelength of 535 nm and results were expressed in terms of ROS levels as percent control (b). Protective effect of 3-O-methyl quercetin and kaempferol on H₂O₂ induced mitochondrial membrane potential. After treatment, cells were incubated with Rhodamine 123 (10 μg/ml) for 1 h at 37 °C and change in fluorescence was measured using a fluorimeter (c). Densitometric analysis of MMP change in lung and liver cells (d). After treatment, total LDH activity in lung (e) and liver (f) cells was determined as described in materials and methods and results were expressed as percent control. Each value represents mean ± SE of three independent experiments. The values were significant at p < 0.05