Fig. 3
CD133+ SFCs from NCI-H446 cell line exhibited higher in vivo tumorigeic capacity compared to that of parental cells. a, In vivo tumorigeic capacity was compared between SFCs and parental NCI-H446 cells. Up: Balb/c-nu mice were inoculated subcutaneously with 1.0 × 105 parental cells in the left fore leg, and no xenograft was observed 4 weeks after inoculation. Down: Balb/c-nu mice were inoculated subcutaneously with 1.0 × 103 SFCs in the right fore leg, and xenograft formed 4 weeks after inoculation. b, The transplanted tumors derived from SFCs and parental cells exhibited the similar histological morphology(HE staining) (100 × magnification). c, The expression levels of stem cell markers (CD133 and CD44) were compared between xenografts formed by SFCs and parental NCI-H446 cells (100 × magnification)