Fig. 5

The effect of cumulative addition of Fr. III (1–10 μg/mL, a) and compounds (1–6, 10–100 μM, b and c) on phenylephrine (PE 10 μM) pre-contracted isolated aortae. Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME, 100 μM), methylene blue (MB, 5 μM), tetraethylammonium chloride (TEA, 10 mM) and indomethacin (INDO, 5 μM) or the vehicle (0.1 % DMSO) were added 30 min before investigating the vasodilator effect. ^*P < 0.05, compared with the corresponding control values; ^#P < 0.05, compared with the corresponding compound values; by Tow Way ANOVA and Dunnett’s post hoc test