Skip to main content
Fig. 8 | BMC Complementary and Alternative Medicine

Fig. 8

From: Mechanisms of Heshouwuyin in regulating apoptosis of testicular cells in aging rats through mitochondrial pathway

Fig. 8

Immunofluorescence and western blot for caspase-3 and statistical analysis. Immunofluorescence staining was used to detect the expression of caspase-3 and the rates of positive cells were quantified(I,III). The results showed that the rates of cells positive for caspase-3 were significantly higher in the NAG than in the YCG (P < 0.01). In the SWY1G and SWY2G, the rate of cells positive for caspase-3 was significantly lower than in the NAG (P < 0.01). a: young control group (YCG); b: natural aging group (NAG); c: Heshouwuyin group 1 (SWY1G); d: Heshouwuyin group 2 (SWY2G). Western blot was used to detect the content of caspase-3 protein and statistical analysis was performed according to the gray level(II,IV). The results showed the variation trend was consistent with the results of immunofluorescence.1: young control group (YCG); 2: natural aging group (NAG); 3: Heshouwuyin group 1 (SWY1G); 4: Heshouwuyin group 2 (SWY2G). Each histogram represents the mean ± SD (n = 3). Columns with different letters represent significantly different values, while the same letters indicate no significant difference. Bars = 20 μm

Back to article page