Fig. 4

Cell cycles phase in different experimental groups detected by flow cytometry. Cell cycle phases in the different experimental groups were detected by flow cytometry. Statistical analysis showed that the rate of cells in the G1/G0 phase was significantly higher and that the rate of cells in the G2/M and S phases was significantly lower in the NAG than in the YCG (P < 0.05). The rate of cells in the G1/G0 phase was significantly lower and the rate of cells in the G₂/M and S phases was significantly higher in the SWY1G and SWY2G than in the NAG (P < 0.05). YCG: young control group; NAG: natural aging group; SWY1G: Heshouwuyin group 1; SWY2G: Heshouwuyin group 2. PI, proliferation index. Each histogram represents the mean ± SD (n = 5). Columns with different letters represent significantly different values, while the same letters indicate no significant difference