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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: The role of kaempferol-induced autophagy on differentiation and mineralization of osteoblastic MC3T3-E1 cells

Fig. 4

Effect of kaempferol on osteogenesis-associated factors. a The cells were treated with kaempferol (1–10 μM) for 24 h. Western blotting showed that kaempferol activated the osteogenic proteins such as RUNX-2, osterix, BMP-2 and collagen I. β-actin was used as the internal control. b The total RNA was extracted from the cultures to perform quantitative PCR. The mRNA expression of osteogenesis-associated genes, including BMP-2, ALP and collagen I, increased in a dose-dependent manner. The data are expressed as the mean ± SD (n=6). The statistical significance of each group was analyzed by a one-way ANOVA and Tukey’s HSD test (*p < 0.05 [BMP-2], **p < 0.001 [BMP2], #p < 0.001 [ALP], and †p < 0.001 [collagen I] for the difference between the treatment group compared to the control group)

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