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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Ethanolic extract of Schizonepeta tenuifolia attenuates osteoclast formation and activation in vitro and protects against lipopolysaccharide-induced bone loss in vivo

Fig. 2

EEST down-regulates RANKL-mediated phosphorylation of Akt and IkB. a BMMs were cultured for 1 day in the presence of M-CSF (10 ng/mL). Next, BMMs were starved for 3 h, pretreated with EEST (50 μg/mL) for 1 h and then stimulated with RANKL (50 ng/mL) for the indicated times. Cell lysates were analyzed by western blotting with antibodies against p-p38, p38, p-ERK, ERK, p-JNK, JNK, p-Akt, Akt, p-IκB, IκB, and GAPDH. b BMMs were infected with retroviruses expressing pMX-IRES-EGFP (pMX), pMX- CA-IKKβ-EGFP, and pMX-Akt-EGFP. Infected BMMs were cultured with or without EEST (50 μg/mL) in the presence of M-CSF (30 ng/mL) and RANKL (50 ng/mL) for 4 days. After culturing, the cells were fixed and stained for TRAP (left). The TRAP+ MNCs with more than 5 nuclei were counted (right). *P < 0.05, **P < 0.01 vs. the indicated group

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