Optimization of extraction parameters of PTP1β (protein tyrosine phosphatase 1β), inhibitory polyphenols, and anthocyanins from Zea mays L. using response surface methodology (RSM)

Table 1 Inhibitory effect of the crude extract and fractions of Zea mays L. on protein tyrosine phosphatase 1β

Extract and fractions	Concentration (μg/mL)	Inhibition (%)	IC₅₀^b (μg/mL)
Suramin^a	12.97	61.88
	6.49	40.62	7.51
	3.21	10.69
EtOH ext.	100	43.99	-
n-Hex fr.	100	25.54	-
CH₂Cl₂ fr.	100	28.12	-
EtOAc fr.	100	83.88
	50	57.23	26.12
	10	44.77
n-BuOH fr.	100	70.89
	50	45.09	58.20
	10	26.87	-
Water fr.	100	47.50	-

^aSuramin was used as positive control
^bThe IC₅₀ value was defined as the half-maximal inhibitory concentration and mean of 3 duplicate analyses of each sample

ISSN: 2662-7671