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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: A water extract of Malva verticillata seeds suppresses osteoclastogenesis and bone resorption stimulated by RANK ligand

Fig. 2

WEMV does not affect osteoblast differentiation. C2C12 cells were cultured in differentiation medium with the indicated concentrations of WEMV for 3 days. a ALP activity of C2C12 cells was determined using an ALP assay with naphthol AS-BI phosphate as described in the Methods. b A CCK-8 assay was performed to determine the viability of C2C12 cells after a 2-day incubation with the indicated concentrations of WEMV. Calvarial osteoblast cells were cultured in differentiation medium for 15 days. c ALP activity and (d) calvarial osteoblast cell viability were examined after 15- and 2-day incubations, respectively

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