Fig. 2From: A water extract of Malva verticillata seeds suppresses osteoclastogenesis and bone resorption stimulated by RANK ligandWEMV does not affect osteoblast differentiation. C2C12 cells were cultured in differentiation medium with the indicated concentrations of WEMV for 3 days. a ALP activity of C2C12 cells was determined using an ALP assay with naphthol AS-BI phosphate as described in the Methods. b A CCK-8 assay was performed to determine the viability of C2C12 cells after a 2-day incubation with the indicated concentrations of WEMV. Calvarial osteoblast cells were cultured in differentiation medium for 15 days. c ALP activity and (d) calvarial osteoblast cell viability were examined after 15- and 2-day incubations, respectivelyBack to article page