Fig. 4

Effects of ZMHE on eotaxin-1 gene expression. a The eotaxin-1 luciferase reporter vector was transfected into NIH/3 T3 cells and cultured for 24 h. The cells were pretreated with ZMHE for 1 h and then stimulated with IL-4. Luciferase activity was calculated against IL-4-unstimulated control. b Cells were pretreated with ZMHE for 1 h and then further incubated with IL-4 (50 ng/ml) for 24 h. Eotaxin-1 release was then determined using an ELISA. The results are mean ± standard deviation (SD) (n = 3). P < 0.01 vs. IL-4-untreated control. *P < 0.05 vs. IL-4-treated control. **P < 0.01 vs. IL-4-treated control