Fig. 3

Effects of GJD on the expression of p-JNK and p-38 MAPK in HCT116 cells. a HCT116 cells were treated with 0.3 mg/ml GJD for different times (0, 0.5, or 2 h), after which Akt, p-Akt, ERK1/2, p-ERK1/2, c-Jun, p-c-Jun, p-JNK, and p-p38 levels were examined by western blotting. b HCT116 cells were pretreated with 0.3 mg/ml GJD for 1 h before exposure to 100 nM PMA for 15 min. Whole cell lysates were then subjected to immunoblot analysis as in (a)