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Table 2 Zone of inhibition (in mm) of the different concentrations of crude and solvent fractions of the leaves of Rhamnus prinoides against gram negative bacteria

From: Evaluation of the in vitro antibacterial activity of the solvent fractions of the leaves of Rhamnus prinoides L’Herit (Rhamnaceae) against pathogenic bacteria

Category of test

Concentration

Bacteria

S. flexneri

E. coli

Stand

Clinic

Stand

clinic

Crude

19.5 mg/well

12.00 ± 0.58a3d2

11.67 ± 0.33 a3d2

11.67 ± .88a3d1

11.33 ± 0.67a3d2

39 mg/well

14.00 ± 0.00a3d1

13.67 ± 0.33 a3d1

12.67 ± 0.33a3

12.67 ± 0.33a3

78 mg/well

17.00 ± 0.58a3

16.00 ± 0.58 a3

15.00 ± 0.00a3

14.50 ± 0.29 a3

Cef 0.03 mg/disc*

NT

NT

28.33 ± 0.88

27.00 ± 0.58

Cip 0.005 mg/disc*

26.33 ± 0.67

25.00 ± 0.58

NT

NT

Methanol

19.5 mg/well

11.00 ± 0.00a3d2

10.67 ± 0.67a3d2

10.33 ± 0.33a3d3

9.00 ± 0.58a3c2d3

39 mg/well

13.00 ± 0.58a3

12.67 ± 0.33a3

12.00 ± 0.58a3d1

11.67 ± 0.33a3d2

78 mg/well

15.00 ± 0.58a3

14.67 ± 0.33a3

14.50 ± 0.29a3

14.33 ± 0.33a3

Cef 0.03 mg/disc*

NT

NT

27.67 ± 0.33

26.67 ± 0.33

Cip 0.005 mg/disc*

27.00 ± 0.58

26.33 ± 0.67

NT

NT

Chloroform

19.5 mg/well

10.00 ± 0.58a3d2

10.33 ± 0.33a3

---

---

39 mg/well

11.33 ± 0.33a3f1

11.67 ± 0.67a3

----

---

78 mg/well

13.33 ± 0.33 a3g2

12.33 ± 0.33a3g2

---

---

Cip 0.005 mg/disc*

25.00 ± 0.58

24.67 ± 0.33

NT

NT

  1. Values are expressed as Mean ± S.E.M (n = 3), analysis was performed with One-Way ANOVA followed by Tukey test acompared positive control, bto 19.5 mg/well, cto 39 mg/well, dto 78 mg/well, eto crude 19.5 mg/well, fto crude 39 mg/well, gto crude 78 mg/well; 1P < 0.05, 2P < 0.01, 3P < 0.001. The negative control has shown no antibacterial activity. * = positive controls, Stand = standard (ATCC) strains whereas Clinic. = clinically isolated strains, NT = not tested, Cef = Cefoxitin, Cip = Ciprofloxacin, --- = no activitiy