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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study

Fig. 1

Isolation and characterization of human tendon stem cells (hTSCs). a Schematic representation of the protocol used to isolate hTSCs. b In vitro differentiation of hTSCs toward the adipogenic, the osteogenic, and the chondrogenic phenotypes. Lipid intracellular droplets (red) in the adipocytes were stained with Oil Red O solution. Alizarin Red-S staining revealed the presence of calcium deposits 2016 (yellowish-brown). Alcian Blue staining detected/assessed the proteoglycan content. Typical results are shown. Original magnification x10. c Gene expression of stem cell marker (Oct4, KLF4, and Nanog) by Real-Time PCR in hTSCs and human dermal fibroblasts (hDFs). Data are expressed as means ± SD of three different experiments

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