Fig. 2

Effect of the aqueous extract from the fruits of Hancornia speciosa, rutin and chlorogenic acid in carrageenan-induced peritonitis model. BALB/c mice were treated (i.v.) with saline, aqueous extract at doses of 20, 30 or 40 mg/kg, rutin, chlorogenic acid at the dose of 2, 2.5 or 5 and with dexamethasone (i.p.) at the dose of 0.5 mg/kg, thirty minutes after carrageenan (1 mg/mL) or saline (1 mL) administration (i.p). After 4 h, peritoneal lavage with saline was performed and leucocytes count was performed using a Neubauer chamber (a). The supernatants were collected for the determination of IL-1β (b), IL-6 (c), IL-12 (d), and TNF-α (e), which was performed using an enzyme-linked immunosorbent assay. The solid column (black bar) represents the groups that received saline solution (i.v.) followed by intraperitoneal administration of carrageenan 30 min later. Each column represents the mean of the values obtained from five animals, and the vertical lines indicate the standard errors of the mean (SEM). ^###p < 0.001 compared with the group treated with saline; ***p < 0.001 compared with the carrageenan group. Sal: saline (0.9 mg/mL); Dx: dexamethasone (0.5 mg/kg)