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Pharmacological screening of Monotheca buxifolia (Falc.) A. DC. for antinociceptive, anti-inflammatory and antipyretic activities
© The Author(s). 2016
Received: 28 February 2016
Accepted: 2 August 2016
Published: 5 August 2016
Medicinal plants have proven their importance as a valuable source of molecules with therapeutic potential. Monotheca buxifolia (Falc.) A. DC. (family: Sapotaceae) is traditionally used as a hematinic, laxative, digestive, anthelmintic, antipyretic, and in the treatment of gastro-urinary disorders. To provide scientific evidence for its folkloric use, the present study investigated Monotheca buxifolia fruit hydro-ethanolic extract (MBHE) for its prospective antinociceptive, anti-inflammatory and antipyretic activities. MBHE was eluted through column chromatography to isolate the bioactive secondary metabolites which may probably involve in its beneficial properties.
The phytochemical constituents in MBHE was elucidated using UV, IR, 1H-NMR, 13C NMR, 2D-NMR spectra in combination with EIMS and FAB-MS spectrometric techniques and comparison with literature data of related compounds. The antinociceptive activity of MBHE was evaluated in the acetic acid induced abdominal constriction assay; the anti-inflammatory potential was assessed in the carrageenan induced paw edema, while the antipyretic effect was tested against brewer’s yeast induced pyrexia in BALB/c mice at doses of 50, 100 and 150 mg/kg.
Elution of MBHE along with various characterization techniques led to the isolation of oleanolic acid and isoquercetin. Significant attenuation of chemical induced nociception was observed with MBHE at tested doses of 50 mg/kg (P < 0.01, 68.87 %), 100 mg/kg (P < 0.01, 68.87 %) and 150 mg/kg (P < 0.001, 83.02 %). During a duration of 1–5 h in the carrageenan induced paw edema assay, significant ameliorative effect (P < 0.01, P < 0.001) was demonstrated by MBHE at 50 mg/kg (22.94–20 %), 100 mg/kg (33.23–21.13 %) and 150 mg/kg (38.23–25 %). MBHE also significantly alleviated the brewer’s yeast induced pyrexic response when tested at doses of 50 mg/kg (P < 0.05 in 2nd h), 100 mg/kg (P < 0.05, P < 0.01 and P < 0.001 in 1–5 h) and 150 mg/kg (P < 0.01 and P < 0.001 in 1–5 h).
These findings suggest that Monotheca buxifolia possess pain, inflammation and pyrexia ameliorating properties, probably mediated by the presence of oleanolic acid and isoquercetin contents, though the involvement of other important phytochemicals constituents cannot be ignored.
KeywordsSapotaceae Oleanolic acid Isoquercetin Pain Inflammation Pyrexia
Discovering excellent remedies for diseases that are efficacious, economical and having minimum adverse effects is the need of the hour. For discovering such products, medicinal plants are considered as best choice, as they provide a wide range of bioactive compounds, making them a rich source of different types of medicines . Majority of drugs currently used in the clinics are due to extensive research on isolation from the natural sources .
Monotheca buxifolia (Falc.) A. DC. is a member of genus Monotheca which belongs to family Sapotaceae. M. buxifolia is one of the important tree species of Pakistan that still exhibits dominance in some of the forests, particularly in the Dir District. It is also distributed in the mountains of Afghanistan, northern Oman, and in the south-east Saudi Arabia. M. buxifolia is mainly used for fuel, fodder, small timber, roof thatching materials, and notably used as fence around cultivated fields due to its thorny nature. This species also yields fruits, locally called Gurguri, which provides a source of income for the local inhabitants . In folk medicine, M. buxifolia fruit is used as hematinic, laxative, purgative, vermicidal, antipyretic, and for the management of gastro-urinary disorders [4–7]. The leaves of M. buxifolia contain anthraquinones, flavonoids, terpenoids, cardiac glycosides, saponins, reducing sugars, tannins and poly-phenolic compounds . The total phenolic compounds in M. buxifolia fruit fractions, ranged between 59.13 ± 2.6 mg and 16.66 ± 1.3 mg/g dry weight of fraction with the butanolic extract showed the highest total phenolics (59.13 ± 2.6 mg GAE/g fraction). Likewise, the flavonoid contents were in the range of 4.11 ± 0.51 to 48.68 ± 2.8 mg as rutin equivalents/g fraction with highest amount observed in the aqueous fraction (48.68 ± 2.8 mg/g) . Recently, two new compounds, buxifoline-A as alkaloid and buxilide as pyrone were isolated from the ethylacetate fraction of M. buxifolia fruit . Flavonoids and poly-phenolic compounds are reported to possess potent anti inflammatory and analgesic properties. Previously, the in vitro antioxidant activity of this fruit has been evaluated and has proved to exhibit potent antioxidant properties . Moreover, M. buxifolia fruit also possess inhibitory potential against urease enzyme . The family Sapotaceae is widely studied for antimicrobial [10, 11], antioxidant , antipyretic , CNS depressant , anti-inflammatory [14, 15], anthelmintic  and antinociceptive activities [12, 17] in various in vitro and in vivo experimental models.
Scientific studies concerning the therapeutic efficacy of M. buxifolia are lacking and, in an attempt to provide scientific evidence, we tested the M. buxifolia fruit hyrdo-ethanolic (30:70) extract (MBHE) for antinociceptive, anti-inflammatory and antipyretic activities in mice.
Chemicals and drugs
Tramadol (Tramal®, Searle Company (Pvt), Pakistan), diclofenac sodium (Voren®, AsianContinental (Pvt), Pakistan), carrageenan and brewer’s yeast (Sigma-Aldrich).
BALB/c mice of either sex (21–35 g) were purchased from the animal house of the Department of Pharmacy, University of Peshawar and were acclimatized at 25 ± 2 °C under a 12 h dark/light cycle for 10 days. Food and water were provided ad libitum. The experimental protocols for this study were approved by the Ethical Committee of the Department of Pharmacy, University of Peshawar, Pakistan (registration number: 04/EC-15/Pharm).
Fruits of Monotheca buxifolia were collected from the northern areas (District Dir Lower) of Pakistan in August 2013. It was identified by Ghulam Jelani, taxonomist at the Department of Botany, University of Peshawar. Afterwards, a specimen was deposited in the herbarium under the voucher number Bot. 20061 (PUP).
Preparation of Monotheca buxifolia fruit extract
Fruits of Monotheca buxifolia (50 kg) were collected and washed to remove dust and other impurities. The seeds were separated and the collected fleshy fruit pulp was dried under shade at ambient temperature. The dried pulp (6.5 kg) was coarsely grinded and was subjected to extraction by adding hydro-ethanolic solvent (40 L) with occasional shaking for 14 days (2 × 7) according to a previous reported method . It was then filtered using a Whatman-1 filter paper. The solvent was evaporated under reduced pressure in a rotary evaporator (BUCHI Rotavapor R-200, Switzerland) at 40 °C until a semisolid mass (2.419 kg) was obtained (yield 4.838 %). The obtained extract was kept in refrigerator till further analysis.
MBHE was preliminary evaluated by qualitative phytochemical analysis  and was further screened by quantitative analysis of phenolic, flavonoid  and triterpenoid  contents. MBHE was also subjected to different analytical techniques for isolation and structural elucidation of natural compounds. MBHE (2.304 kg) was mixed with 2.5 L distilled water and soaked overnight, extracted successively with hexanes (3 × 5 L), chloroform (3 × 5 L), ethyl-acetate (3 × 5 L), and n-butanol (3 × 5 L) to obtain hexane soluble (56 g), chloroform soluble (57.7 g), ethylacetate soluble (34.9 g) and n-butanol soluble (54.5 g) fractions respectively. The ethyl-acetate fraction (30 g) was subjected to vacuum liquid chromatography on normal phase silica gel and eluted using hexane, hexanes-ethylacetate, ethylacetate, ethylacetate-methanol and methanol with increasing polarity to yield 18 fractions (Fr. 1–18). Compound 1 (13 mg) was obtained from fraction 2 (1.2 g) through repeated column chromatography using hexanes-ethylacetate (9:1 – 8:2) while fraction 15 (1.4 g) was re-chromatographed on silica gel column using hexanes-ethylacetate (8:2) to obtained compound 2 (15 mg). Purity of these two compounds was assessed using TLC followed by spraying with ceric sulphate and heating. Their chemical structure was elucidated using 1H-NMR, 13C-NMR, 2D-NMR, EI-MS, FAB-MS, UV, and IR analytical techniques. The data obtained for these compounds were unambiguously matched with reported data from the literature [22, 23].
Where A is the paw volume of carrageenan alone treated group and B is the paw volume of tested group.
The antipyretic potential of MBHE was screened against brewer’s yeast induced pyrexia  in BALB/c mice (30–35 g). The animals were deprived of food for 5 h before the start of experiment. Their normal rectal temperature was recorded using a digital thermometer, after which a 15 % solution of brewer’s yeast was injected subcutaneously at a dose of 10 ml/kg to each animal. The rise in body temperature was recorded after 24 h and animals showing at least 0.5 °C rise of their body temperature were included in the experiment. The pyrexic animals were orally administered with MBHE at doses of 50, 100 and 150 mg/kg or acetaminophen, which was used as standard in a dose of 150 mg/kg. The rectal temperature of animals was then recorded at 1 to 5 h duration. The mean elevation of body temperature in °F across treated groups was compared to that of untreated control.
Data were expressed as mean ± S.E.M or SD. Statistical analysis was performed by one way ANOVA followed by Dunnett’s or Tukey’s post hoc test where appropriate using GraphPad Prism 5 (GraphPad Software Inc. San Diego CA, USA). Statistical significance was deduced at P ≤ 0.05.
Phytochemical analysis of Monotheca buxifolia
Qualitative phytochemical analysis of Monotheca buxifolia
Ferric chloride test
Lead acetate test
Ethyl acetate test
Sodium hydroxide test
Monotheca buxifolia attenuates tonic visceral chemical induced nociception
Monotheca buxifolia alleviates carrageenan induced paw edema
Anti-inflammatory activity of Monotheca buxifolia hydro-ethanolic extract (MBHE) in carrageenan induced paw edema in mice
0.235 ± 0.012
0.227 ± 0.026
0.240 ± 0.011
0.210 ± 0.016
0.230 ± 0.036
0.340 ± 0.016###
0.370 ± 0.028###
0.395 ± 0.025###
0.490 ± 0.057###
0.440 ± 0.028###
Aspirin (150 mg/kg)
0.255 ± 0.028**
0.250 ± 0.035***
0.252 ± 0.020***
0.325 ± 0.052***
0.305 ± 0.019***
(MBHE 50 mg/kg)
0.262 ± 0.022**
0.282 ± 0.012**
0.287 ± 0.025***
0.292 ± 0.015***
0.352 ± 0.029**
(MBHE 100 mg/kg)
0.227 ± 0.040***
0.277 ± 0.035**
0.290 ± 0.046***
0.287 ± 0.037***
0.347 ± 0.029**
(MBHE 150 mg/kg)
0.210 ± 0.028***
0.257 ± 0.026***
0.287 ± 0.022***
0.272 ± 0.044***
0.330 ± 0.038**
Monotheca buxifolia relieves brewer’s yeast induced pyrexia
Antipyretic activity of Monotheca buxifolia hydro-ethanolic extract (MBHE) in brewer’s yeast induced pyrexia in mice
(Brewer’s yeast + Saline)
100.4 ± 0.358
100.5 ± 0.346
100.0 ± 0.335
100.4 ± 0.265
100.5 ± 0.341
(Acetaminophen 150 mg/kg)
96.18 ± 0.757***
96.62 ± 0.755***
95.50 ± 1.023***
96.07 ± 0.720***
95.23 ± 0.731***
(MBHE 50 mg/kg)
97.93 ± 0.449
98.20 ± 0.634*
98.58 ± 0.838
99.27 ± 0.860
99.88 ± 0.812
(MBHE 100 mg/kg)
96.02 ± 0.884***
97.18 ± 0.570**
97.68 ± 0.244*
97.72 ± 0.342*
97.42 ± 0.709*
(MBHE 150 mg/kg)
93.70 ± 0.753***
96.93 ± 0.584***
96.48 ± 0.303**
97.33 ± 0.547**
96.35 ± 0.837**
Fruit of Monitica buxifollia was screened for its potential antinociceptive, anti-inflammatory, and antipyretic activities. The nociceptive response in the acetic acid induced writhing test results from the production of prostaglandins through the action of cyclooxygenases. The liberated prostaglandins stimulate sensory pathways in the mouse peritoneum and incite viscero-somatic y reflexes manifested as strong abdominal constrictions or writhes. The acetic acid induced writhes are sensitive to various analgesics. MBHE when administered at doses of 50, 100 and 150 mg/kg depressed the acetic acid induced writhes and the antinociceptive effect was analogous to that of the standard diclofenac sodium (50 mg/kg).
Carrageenan-induced mice paw edema assay is a widely used test to determine the anti-inflammatory activity of both natural and synthetic compounds. Edema formation due to carrageenan administration in mouse paw is a biphasic event. The initial phase lasting about 1–5 h is predominately characterized by a non-phagocytic edema and has been attributed to the action of various mediators including histamine, serotonin and bradykinin on vascular permeability . The initial phase is followed by a second phase having a duration of 2–5 h and results from overproduction of prostaglandins . It has been reported that the second phase of edema is sensitive to drugs like hydrocortisone, phenylbutazone and indomethacin. In the present study, we have observed potential anti-inflammatory activity of MBHE at all the tested doses (50, 100 and 150 mg/kg) and was comparable to the classical cyclooxygenase inhibitor, aspirin (150 mg/kg).
Brewer’s yeast is an exogenous pyrogen which produces pathogenic fever by binding to lipopolysaccharide binding protein and results in the release of different cytokines like interleukin 1, 6, tumor necrosis factor alpha and prostaglandins. These pro-inflammatory mediators cross the blood brain barrier and act on hypothalamus causing the release of prostaglandin E2 which is produced through the action of cyclo-oxygenase-2 and thus increases the body temperature . In this study, potential antipyretic effect was observed for the 100 and 150 mg/kg doses of MBHE and the effect was similar to that of acetaminophen (150 mg/kg).
Biologically active molecules derived from herbal extracts can be used to treat acute and chronic diseases . In the current study, the chromatographically isolated compounds i.e. isoquercetin have been reported to possess neuroprotective , cardioprotective , hepatoprotective , nephroprotective , chemopreventive , analgesic , antioxidant  and anti-inflammatory  properties. In addition, oleanolic acid has anti-tumor , anti-inflammatory , antioxidant , anti-hyperlipidemic , anti-ulcer , anti-microbial  and analgesic [45, 46] activities. Keeping in view, the potent antinociceptive, anti-inflammatory, and antipyretic activity of MBHE, it can be argued that these beneficial effects can be attributed at least to the presence of isoquercetin and oleanolic acid contents in M. buxifolia. However, the involvement of other phytochemical constituents cannot be ignored in the mediation of pain, inflammation and pyrexia relieving properties as M. buxifolia has been reported to contain high amount of phenolics and flavonoids , β-sitosterol , tannins, tri-terpenoids, glycosides, anthraquinones and alkaloids  which is further corroborated in this study. Flavonoids are polyphenolic compounds that occur ubiquitously in plants and having a variety of biological effects including antioxidant, anti-inflammatory, antihypertensive, anti-atherosclerotic, anti-tumor, anti-thrombogenic, anti-osteoporotic, antimicrobial, antiviral, anti-ulcerogenic, and antihepatotoxic activities [49, 50]. Naturally occurring anthraquinones are a group of secondary metabolites that possess anti-inflammatory, anti-cancer, antidiabetic, antimicrobial and laxative properties . Similarly, other phytochemical constituents like glycosides and alkaloids also have known beneficial pharmacological effects both in vitro and in vivo [52, 53].
Monotheca buxifolia hydroethanolic extract attenuated the tonic visceral chemical induced nociception and alleviated a phlogistic agent induced inflammatory response. It also relieved a pyrogenic substance induced elevation of body temperature. Phytochemical analysis revealed that M. buxifolia contained isoquercetin and oleanolic acid that might be involved in allay of pain, inflammation and pyrexia. Though, the involvement of other phytochemical constituents cannot be ignored in mediating the antinociceptive, anti-inflammatory and antipyretic effects, as M. buxifolia also have sufficient amount of flavonoids, anthraquinones glycosides, triterpenoids, and alkaloids. Our findings suggest that M. buxifolia is able to mend disease conditions afflicted with pain, inflammation and pyrexia. Further studies are warranted like HPLC-DAD profile to elucidate the exact chemical constituents responsible for these beneficial pharmacological effects.
The authors are grateful to the H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan and COMSATS Institute of Information Technology, Abbottabad, Pakistan for providing necessary facilities related to phytochemical analysis.
The authors have not received any funding for this study.
Availability of data and materials
All data and materials related to this study are available with the corresponding author. Moreover, Additional file 1 is available along with the manuscript.
IU conducted the experiments and prepared the initial draft of manuscript. JAK guided the research group as supervisor throughout the research project. MS helped in the analysis and interpretation of data as well as in preparing the final version of manuscript. AK assisted in the analytical work. AA helped in the structure elucidation of compounds. PAH provided his help in the collection and extraction procedures. IJ assisted in manuscript writing. FS attributed necessary experimental materials. UF performed qualitative and quantitative analysis of MBHE. All authors read and approved the final manuscript.
The authors declare that they have no competing interest.
Consent for publication
Ethics approval and consent to participate
The experimental procedures on laboratory animals were approved by the Ethical Committee of the Department of Pharmacy, University of Peshawar, Pakistan (04/EC-15/Pharm).
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- Munuswamy H, Thirunavukkarasu T, Rajamani S, Elumalai EK, Ernest D. A review on antimicrobial efficacy of some traditional medicinal plants in Tamilnadu. J Acute Dis. 2013;2(2):99–105.View ArticleGoogle Scholar
- Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109 Suppl 1:69.View ArticlePubMedPubMed CentralGoogle Scholar
- Khan N, Ahmed M, Shaukat SS, Wahab M, Siddiqui MF. Structure, diversity, and regeneration potential of Monotheca buxifolia (Falc.) A. DC. dominated forests of Lower Dir District, Pakistan. Front Agric China. 2011;5(1):106–21.View ArticleGoogle Scholar
- Shah AM SK, Gohar F, Khan A, Bhatti KH, Amin M, Din NU, Ahmad M, Zafar M. Ethnobotanical study of medicinal plants of semi-tribal area of Makerwal & Gulla Khel (lying between Khyber Pakhtunkhwa and Punjab Provinces), Pakistan. Am J Plant Sci. 2013;4:98–116.View ArticleGoogle Scholar
- Ullah R, Hussain Z, Iqbal Z, Hussain J, Khan FU, Khan N, et al. Traditional uses of medicinal plants in Darra Adam Khel NWFP Pakistan. J Med Plants Res. 2010;17:1815–21.Google Scholar
- Rehman J, Khan IU, Farid S, Kamal S, Aslam N. Phytochemical screening and evaluation of in-vitro antioxidant potential of Monotheca buxifolia. E3 J Biotechnol Pharm Res. 2013;4(4):54–60.Google Scholar
- Murad W, Azizullah A, Adnan M, Tariq A, Khan KU, Waheed S, et al. Ethnobotanical assessment of plant resources of Banda Daud Shah, District Karak, Pakistan. J Ethnobiol Ethnomed. 2013;9(1):1.View ArticleGoogle Scholar
- Jan S, Khan MR, Rashid U, Bokhari J. Assessment of antioxidant potential, total phenolics and flavonoids of different solvent fractions of Monotheca Buxifolia fruit. Osong Public Health Res Perspect. 2013;4(5):246–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Ullah I, Khan JA, Adhikari A, Khan A, Hannan PA, Wadood A, et al. Bioassay- guided isolation of new urease inhibitory constituents from Monotheca buxifolia (Falc.) fruit and their molecular docking studies. Rec Nat Prod. 2016;10(6):744–9.Google Scholar
- Kuete V, Tangmouo JG, Penlap Beng V, Ngounou FN, Lontsi D. Antimicrobial activity of the methanolic extract from the stem bark of tridesmostemon omphalocarpoides (Sapotaceae). J Ethnopharmacol. 2006;104(1–2):5–11.View ArticlePubMedGoogle Scholar
- Marwat SK, Rehman F, Usman K, Khakwani A, Ghulam S, Anwar N, et al. Medico-ethnobotanical studies of edible wild fruit plants species from the flora of north western Pakistan (DI Khan district). J Med Plants Res. 2011;5:3679–86.Google Scholar
- Shekhawat N, Vijayvergia R. Investigation of anti-inflammatory, analgesic and antipyretic properties of Madhuca indica GMEL. Int J Mol Med Adv Sci. 2010;6(2):26–30.Google Scholar
- Nasrin M, Dash PR, Saha MR. Investigation of analgesic and neuropharmacological activities of methanolic bark extract of Mimusops elengi. Int J Pharm Sci Res. 2011;2:2050–5.Google Scholar
- Araujo-Neto V, Bomfim RR, Oliveira VO, Passos AM, Oliveira JP, Lima CA, et al. Therapeutic benefits of Sideroxylon obtusifolium (Humb. ex Roem. & Schult.) TD Penn., Sapotaceae, in experimental models of pain and inflammation. Rev Bras Farmacogn. 2010;20(6):933–8.View ArticleGoogle Scholar
- Purnima A, Koti B, Thippeswamy A, Jaji M, Swamy AV, Kurhe Y, et al. Antiinflammatory, analgesic and antipyretic activities of Mimusops elengi Linn. Indian J Pharm Sci. 2010;72(4):480.View ArticlePubMedPubMed CentralGoogle Scholar
- Koné WM, Vargas M, Keiser J. Anthelmintic activity of medicinal plants used in Côte d’Ivoire for treating parasitic diseases. Parasitol Res. 2012;110(6):2351–62.View ArticlePubMedGoogle Scholar
- Karmakar UK, Sultana R, Biswas NN. Antioxidant, analgesic and cytotoxic activities of Mimusops elengi Linn. leaves. Int J Pharm Sci Res. 2011;2(11):2791.Google Scholar
- Muhammad N, Saeed M, Khan H. Antipyretic, analgesic and anti-inflammatory activity of Viola betonicifolia whole plant. BMC Complement Altern Med. 2012;12(1):59.View ArticlePubMedPubMed CentralGoogle Scholar
- Raaman N. Qualitative phytochemical screening. In: Phytochemical techniques. New India Publishing; 2006. p. 19–24.Google Scholar
- Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med. 2012;12(1):221.View ArticlePubMedPubMed CentralGoogle Scholar
- Wei L, Zhang W, Yin L, Yan F, Xu Y, Chen F. Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities. Electron J Biotechnol. 2015;18(2):88–95.View ArticleGoogle Scholar
- Eldahshan O. Isolation and structure elucidation of phenolic compounds of carob leaves grown in Egypt. Curr Res J Biol Sci. 2011;3(1):52–5.Google Scholar
- Seo S, Tomita Y, Tori K. Biosynthesis of oleanene-and ursene-type triterpenes from [4-13C] mevalonolactone and sodium [1, 2-13C2] acetate in tissue cultures of Isodon japonicus Hara. J Am Chem Soc. 1981;103(8):2075–80.View ArticleGoogle Scholar
- Collier H, Dinneen L, Johnson CA, Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol Chemother. 1968;32(2):295–310.View ArticlePubMedPubMed CentralGoogle Scholar
- Morris CJ. Carrageenan-induced paw edema in the rat and mouse. Inflammation Protocols. USA: Springer; 2003. p. 115–21.Google Scholar
- Gabriel EI, Chidiebere OF, Ota AA. Evaluation of the methanolic rhizome extract of Anchomanes difformis for analgesic and antipyretic activities. Int J Basic Appl Sci. 2013;2(4):289–96.Google Scholar
- Maity TK, Mandal SC, Mukherjee PK, Saha K, Das J, Pal M, et al. Studies on antiinflammatory effect of Cassia tora leaf extract (fam. Leguminosae). Phytother Res. 1998;12(3):221–3.View ArticleGoogle Scholar
- Pérez-Guerrero C, Herrera MD, Ortiz R, de Sotomayor MA, Fernández MA. A pharmacological study of Cecropia obtusifolia Bertol aqueous extract. J Ethnopharmacol. 2001;76(3):279–84.View ArticlePubMedGoogle Scholar
- Math P, Mishra D, Prajapati P, Roshy J, Jha P. Antipyretic activity of madhukadi and madhukadi Ghana-an experimental study. Int J Pharm Biol Arch. 2011;2(1):572–6.Google Scholar
- Cragg GM, Newman DJ. Natural products: a continuing source of novel drug leads. Biochim Biophys Acta. 2013;1830(6):3670–95.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang C-P, Li J-L, Zhang L-Z, Zhang X-C, Yu S, Liang X-M, et al. Isoquercetin protects cortical neurons from oxygen–glucose deprivation–reperfusion induced injury via suppression of TLR4–NF-кB signal pathway. Neurochem Int. 2013;63(8):741–9.View ArticlePubMedGoogle Scholar
- Annapurna A, Reddy CS, Akondi RB, Rao SR. Cardioprotective actions of two bioflavonoids, quercetin and rutin, in experimental myocardial infarction in both normal and streptozotocin-induced type I diabetic rats. J Pharm Pharmacol. 2009;61(10):1365–74.View ArticlePubMedGoogle Scholar
- Gilani A, Janbaz K, Shah B. Quercetin exhibits hepatoprotective activity in rats. Biochem Soc Trans. 1997;25(4):S619-S.View ArticleGoogle Scholar
- Nabavi SM, Nabavi SF, Habtemariam S, Moghaddam AH, Latifi AM. Ameliorative effects of quercetin on sodium fluoride-induced oxidative stress in rat’s kidney. Ren Fail. 2012;34(7):901–6.View ArticlePubMedGoogle Scholar
- Murakami A, Ashida H, Terao J. Multitargeted cancer prevention by quercetin. Cancer Lett. 2008;269(2):315–25.View ArticlePubMedGoogle Scholar
- Qiu X-C, Zhang J, Wang Y, Han J-S, He S-P, Wei M-C. Isoquercitrin increases brain cGMP levels and potentiates electroacupuncture (EA) analgesia. Yao Xue Xue Bao. 1985;20(12):891–5.PubMedGoogle Scholar
- Boots AW, Haenen GR, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol. 2008;585(2):325–37.View ArticlePubMedGoogle Scholar
- Morikawa K, Nonaka M, Narahara M, Torii I, Kawaguchi K, Yoshikawa T, et al. Inhibitory effect of quercetin on carrageenan-induced inflammation in rats. Life Sci. 2003;74(6):709–21.View ArticlePubMedGoogle Scholar
- Hsu H-Y, Yang J-J, Lin C-C. Effects of oleanolic acid and ursolic acid on inhibiting tumor growth and enhancing the recovery of hematopoietic system postirradiation in mice. Cancer Lett. 1997;111(1):7–13.View ArticlePubMedGoogle Scholar
- Singh G, Singh S, Bani S, Gupta B, Banerjee S. Anti-inflammatory activity of oleanolic acid in rats and mice. J Pharm Pharmacol. 1992;44(5):456–8.View ArticlePubMedGoogle Scholar
- Wang X, Ye X-l, Liu R, Chen H-L, Bai H, Liang X, et al. Antioxidant activities of oleanolic acid in vitro: possible role of Nrf2 and MAP kinases. Chem Biol Interact. 2010;184(3):328–37.View ArticlePubMedGoogle Scholar
- Liu J, Sun H, Wang X, Mu D, Liao H, Zhang L. Effects of oleanolic acid and maslinic acid on hyperlipidemia. Drug Dev Res. 2007;68(5):261–6.View ArticleGoogle Scholar
- Rodríguez JA, Astudillo L, Schmeda-Hirschmann G. Oleanolic acid promotes healing of acetic acid-induced chronic gastric lesions in rats. Pharmacol Res. 2003;48(3):291–4.View ArticlePubMedGoogle Scholar
- Hichri F, Jannet HB, Cheriaa J, Jegham S, Mighri Z. Antibacterial activities of a few prepared derivatives of oleanolic acid and of other natural triterpenic compounds. Comptes Rendus Chimie. 2003;6(4):473–83.View ArticleGoogle Scholar
- Vasconcelos MAL, Royo VA, Ferreira DS, Crotti AEM, Carvalho JCT, Bastos JK, et al. In vivo analgesic and anti-inflammatory activities of ursolic acid and oleanoic acid from Miconia albicans (Melastomataceae). Z Naturforsch C. 2006;61(7–8):477–82.PubMedGoogle Scholar
- Szakiel A, Ruszkowski D, Grudniak A, Kurek A, Wolska KI, Doligalska M, et al. Antibacterial and antiparasitic activity of oleanolic acid and its glycosides isolated from marigold (Calendula officinalis). Planta Med. 2008;74(14):1709–15.View ArticlePubMedGoogle Scholar
- Nazir M, Khan S, Bhatty M. The constituents of unsaponifiable from Monotheca buxifolia seed oil. Fette, Seifen, Anstrichmittel. 1986;88(7):266–8.View ArticleGoogle Scholar
- Ullah I, Khan JA, Adhikari A, Shahid M. Hepatoprotective effect of Monotheca buxifolia fruit against antitubercular drugs-induced hepatotoxicity in rats. Bangladesh J Pharmacol. 2016;11(1):248–56.View ArticleGoogle Scholar
- Nijveldt RJ, Van Nood E, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001;74(4):418–25.PubMedGoogle Scholar
- Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K. Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets. 2009;8(3):229–35.View ArticlePubMedGoogle Scholar
- Chien S-C, Wu Y-C, Chen Z-W, Yang W-C. Naturally occurring anthraquinones: chemistry and therapeutic potential in autoimmune diabetes. Evid Based Complement Alternat Med. 2015;2015:357357.View ArticlePubMedPubMed CentralGoogle Scholar
- Pan J, Yuan C, Lin C, Jia Z, Zheng R. Pharmacological activities and mechanisms of natural phenylpropanoid glycosides. Pharmazie. 2003;58(11):767–75.PubMedGoogle Scholar
- Martha Perez Gutierrez R, Maria Neira Gonzalez A, Hoyo-Vadillo C. Alkaloids from piper: a review of its phytochemistry and pharmacology. Mini Rev Med Chem. 2013;13(2):163–93.Google Scholar