Skip to main content

Advertisement

Table 3 Cytotoxicity of plant extracts and doxorubicin towards sensitive and drug-resistant cancer cell lines and normal cells as determined by the resazurin assay after 72 h

From: Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines

Cell lines Samples, IC50 values in μg/mL and degrees of resistancea (in brackets)
Extracts Doxorubicin
AAB AAR ACL ACB
CEM/ADR5000 6.53 ± 0.77 (4.50) 6.52 ± 0.57 (6.65) 22.57 ± 2.17 (2.81) 34.87 ± 3.91 (2.77) 108.00 ± 7.92 (975.60)
MDA-MB-231-pcDNA 5.99 ± 0.3 3.79 ± 0.44 29.51 ± 3.08 25.54 ± 3.01 0.61 ± 0.15
MDA-MB-231-BCRP Degree of resistance 10.30 ± 1.22 (1.72) 8.00 ± 1.01 (2.11) 35.42 ± 3.35 (1.20) 33.60 ± 2.14 (1.32) 4.33 ± 0.26 (7.12)
HCT116 (p53+/+) 5.13 ± 0.64 4.40 ± 0.32 33.15 ± 2.87 29.07 ± 2.68 0.78 ± 0.16
HCT116 (p53−/−) Degree of resistance 9.46 ± 0.88 (1.84) 10.77 ± 0.79 (2.45) >80 (>2.41) >80 (2.75) 2.25 ± 0.04 (2.88)
U87MG 6.29 ± 0.40 8.86 ± 0.69 38.63 ± 3.32 >80 0.59 ± 0.08
U87MG.ΔEGFR Degree of resistance 2.71 ± 0.35 (0.43) 3.43 ± 0.48 (0.39) 32.12 ± 1.19 (0.83) 31.84 ± 2.36 (<0.40) 3.38 ± 0.32 (5.76)
HepG2 Degree of resistance 7.54 ± 0.55 (3.87) 6.84 ± 0.64 (4.26) >80 >80 2.12 ± 0.52 (>37.74)
AML12 29.18 ± 3.03 29.14 ± 2.17 >80 >80 >80
  1. aThe degree of resistance was determined as the ratio of IC50 value in the resistant divided by the IC50 in the sensitive cell line; CEM/ADR5000, MDA-MB-231-BCRP, HCT116 (p53−/−), U87MG.ΔEGFR and AML12 were used as the corresponding resistant counterpart for CCRF-CEM (Table 1), MDA-MB-231-pcDNA, HCT116 (p53+/+), U87MG and HepG2 cells, respectively. The tested methanol extracts were from AAB Albizia adianthifolia bark, AAR Albizia adianthifolia roots, ACL Alchornea cordifolia leaves, ACB Alchornea cordifolia bark. In bold: significant cytotoxic effects. Leukemia CEM/ADR5000 cells were tested in RPMI 1640 medium while carcinoma cells tested using DMEM medium, both containing 10 % FBS and 1 % penicillin-streptomycin