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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Formula PSORI-CM01 eliminates psoriasis by inhibiting the expression of keratinocyte cyclin B2

Fig. 4

Anti-proliferative effect of PSORI-CM01-containing serum on HaCaT cell proliferation in vitro a HaCaT cells without LPS stimulation were incubated with various concentrations of blank or PSORI-CM01-containing serum (8 %, 12 %, 16 % and 20 % by volume) for 24 h, then viability was measured using an MTT assay, analysed by measuring the absorbance at 570 nm. b HaCaT cells stimulated with 10 μg/ml LPS were incubated with 16 % blank or PSORI-CM01-containing serum for 24 h, then viability was measured using an MTT assay (* P < 0.05). c HaCaT cells stimulated with10μg/ml LPS were incubated with 16 % blank or PSORI-CM01-containing serum for 15 h, then viability was measured using a BrdU assay, analysed by measuring the absorbance at 450 nm (* P < 0.05). d HaCaT cells stimulated with10μg/ml LPS were incubated with 16 % blank or PSORI-CM01-containing serum, and then the cell index was measured using the RTCA assay (* P < 0.05, each time point during indicated period)

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