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Table 2 Percentage of live, apoptotic and dead cells after 24 h after exposure to PINO (0.001, 0.01, 0.1 1 10 and 100 μM)

From: Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

 

MDA-MB-231

MCF7

MCF10A

 

Live

Apoptotic

Death

Live

Apoptotic

Death

Live

Apoptotic

Death

Control

83.22 ± 5.33

14.82 ± 4.76

1.95 ± 0.6

92,03 ± 2,69

6,97 ± 2,9

1,26 ± 0,61

98.14 ± 0.47

1.33 ± 0.42

0.5 ± 0.08

0.001 μM

82.17 ± 4.76

15.56 ± 5.56

2.25 ± 0.94

92,38 ± 1,82

6,2 ± 1,83

1,07 ± 0,62

96.17 ± 0.76

2.73 ± 0.48

1.08 ± 0.29

0.01 μM

80.94 ± 8.15

16.24 ± 6.77

2.8 ± 1.57

93,14 ± 2,06

5,98 ± 1,69

0,85 ± 0,42

95.7 ± 1.44

3.45 ± 1.63

0.83 ± 0.37

0.1 μM

76.84 ± 9.32

20.15 ± 8.06

2.99 ± 1.34

92,47 ± 1,33

6,49 ± 1,08

1,03 ± 0,39

95.11 ± 2.6

3.99 ± 2.56

0.88 ± 0.16

1 μM

78.82 ± 7.41

18.05 ± 6.27

3.16 ± 1.29

93,32 ± 1,98

5,86 ± 1,61

0,8 ± 0,43

93.32 ± 1.51

5.85 ± 1.76

0.8 ± 0.25

10 μM

79.33 ± 7.24

17.75 ± 6.15

2.9 ± 1.19

93,89 ± 1,29

5,4 ± 1,08

0,69 ± 0,28

95.07 ± 1.1

4.17 ± 1.3

0.74 ± 0.2

100 μM

78.1 ± 6.84

18.46 ± 5.92

3.42 ± 1.09

91,65 ± 1,25

7,48 ± 1,14

0,85 ± 0,18

91.82 ± 2.33a

7.26 ± 2.54a

0.9 ± 0.23

  1. Values represent the average ± SEM of three independent experiments. a was considered statistically significant respect to the control (p < 0.05)