Fig. 2From: Cnidium officinale extract and butylidenephthalide inhibits retinal neovascularization in vitro and in vivoThe effect of COE and BP on tube formation of human umbilical vein endothelial cells. a Matrigel (400 μL) was added to 24-well plates and allowed to solidify for 1 h at 37 °C. HUVECs were treated with COE and BP in VEGF-containing EGM-2 media and incubated at 37 °C. After 17 h, randomly chosen fields were photographed under light microscope. b The bar graph represents the quantification of tube formation of the control group, the COE-treated and BP-treated groups. The data are presented as the mean ± SE. (n = 3; * p < 0.05 vs. the control group)Back to article page