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Effects of Greenselect Phytosome® on weight maintenance after weight loss in obese women: a randomized placebo-controlled study
© The Author(s). 2016
Received: 9 September 2015
Accepted: 14 July 2016
Published: 22 July 2016
Most subjects regain weight after weight loss due to compensatory adaptations finalized to maintain stable body energy stores. Green tea (GT) preparations, which help maintain energy expenditure while dieting could be a useful strategy to facilitate weight maintenance. The usefulness of GT preparations in weight maintenance has been poorly studied so far with conflicting results. This study evaluated if a supplement of GSP and piperine helps obese women to maintain the weight loss obtained with a 3-month lifestyle intervention.
In a randomized placebo-controlled study, we examined whether a highly bioavailable GT extract may counteract weight regain after weight loss. Forty obese women (age 50.1 ± 10.1 years, Body Mass Index (BMI) 36.3 ± 2.7 kg/m2) underwent a 3-month lifestyle intervention. At the end of the intervention, the women were randomized in two groups for the weight-maintenance phase: 20 of them were prescribed twice a day, for 3 months, with a formula containing 150 mg/dose of Greenselect Phytosome® and 15 mg/dose of pure piperine (GSP group), and 20 were given placebo (P group). Anthropometric measures and body composition were measured before (V-3) and after lifestyle intervention (V0), 1 (V1), 2 (V2), and 3 (V3) months after prescribing supplements and 3 months following the discontinuation of supplements (V6).
Lifestyle intervention induced a significant weight reduction in both groups with similar weight change (−6.2 ± 2.6 in GSP group vs. −4.8 ± 3.1 % in P group). In the GSP group, V1 in comparison to V0, had further reduction in weight and fat mass, which remained stable at V2 and V3 and increased at V6. In the P group, weight and fat mass increased from V2 onwards. Weight changes in GSP group and P group from V0 to V3 were −1.0 kg (95 % CI −2.5 to +0.5) and + 0.3 kg (95 % CI −0.9 to +1.6), respectively.
The proportion of women with weight loss ≥ 5 % was greater in the GSP group than in the P group (75 % vs. 45 % at V1, and 60 % vs. 30 % at V6, p < 0.05 for both groups).
Greenselect Phytosome® devoid of caffeine may have a clinical potential for the maintenance of weight after intentional weight loss.
Clinicaltrials.gov NCT02542449 (September 2015)
Genetic, epidemiological, and physiological studies indicate that body weight is highly regulated, and the increasing prevalence of obesity reflects the interactions of genes favoring energy conservation and storage in an environment that enables access to food calories and a more sedentary lifestyle. Weight loss can be achieved by reducing energy intake and at the same time sustaining energy expenditure. Although weight loss interventions usually lead to weight loss shortly after intervention, majority of subjects once again regain weight after the intervention due to compensatory adaptations finalized to maintain stable body energy stores. The physiological adaptation to weight loss involves several biological pathways mediating the utilization and storage of energy and the regulation of appetite . In both, lean and obese individuals, maintenance of a 10 % or greater reduction in body weight is associated with a decline in 24-hour energy expenditure of approximately 20–25 % . The restraint of the decline in energy expenditure while dieting along with GT preparations could be a useful strategy to facilitate weight maintenance. GT contains a complex mixture of polyphenolic compounds belonging to the family of catechins, mainly epigallocatechin gallate, which are responsible for most of the pharmacological activity of GT. The principal mechanisms of GT are stimulation of fat oxidation through up-regulation of lipid-metabolizing enzymes and an increase in norepinephrine levels and energy expenditure through inhibition of catechol-O-methyltransferase .
The ability of GT preparations assisting weight loss was evaluated in a Cochrane Systematic Review that included 14 randomized controlled trials . This review concluded that the weight loss produced by GT preparations is unlikely to be clinically relevant since it was not statistically significant in majority of the studies. However, the usefulness of GT preparations in weight maintenance has been poorly studied so far with conflicting results .
Despite the wide “nutraceutical” use of GT, pharmacological and clinical data show that these molecules are poorly absorbed orally. Formulation with phospholipids (Phytosome strategy) however, has shown increased absorption with some classes of natural products, including polyphenolics. Thus, the absorption of Greenselect Phytosome® (GSP) is about 3-fold higher compared to the unformulated extract , and GSP was also shown to reduce body weight in subjects with obesity and metabolic syndrome [6, 7]. Currently, a new formulation of GSP combined with piperine, a thermogenic agent, has been developed. This new formulation increases the absorption of various phenolics  and enhances the pharmacodynamics of GSP. This study evaluated whether dietary supplements of GSP and piperine help obese women to maintain the weight loss obtained with a 3-month lifestyle intervention.
The study sample consisted of 40 obese Caucasian women who were recruited from those patients referred to the IRCCS Istituto Auxologico Italiano for a weight-loss lifestyle intervention. Women with uncontrolled hypertension and history of cardiovascular or cerebrovascular events were excluded from the study. Sample size was calculated assuming a ≥5 % weight loss would be the outcome obtained with 3 months of lifestyle intervention. The effective size of the study group was calculated for a binomial test irrespective of weight loss ≥5 %, looking for at least a difference of 25 % between the proportion of women reaching this goal in the placebo and treatment group. We designed our trial with an alpha error of 5 % and a power of 95 %. Calculation was performed using G*Power 3.1, obtaining a sample size of 42 subjects, that was rounded to 20 women for each group.
The Ethics Committee of the Istituto Auxologico Italiano approved the study, and all subjects gave their informed consent after we provided a full explanation of the study.
Lifestyle intervention consisted of weekly individual sessions for nutritional education, advice reinforcement on exercise activity and peer group psychological support. A self-monitor diary, which included details of food consumption, daily physical activity and emotional reactions, was used as a tool for education and reinforcement. Daily caloric requirement was calculated using the Harris-Benedict equation and an individual activity factor. A diet based on a 500-kcal/d deficit from the individual estimated caloric requirement was prescribed. The diet, which was high in vegetables, low in salt and simple sugars consisted of 25 % of total energy intake as protein, 20 % as fat and 55 % as carbohydrate. Fresh foods, at least three fish meals per week and avoiding alcohol, were recommended. The prescribed physical activity program was 210 min per week consisting of 70 % moderate-intensity aerobic physical activity and 30 % muscle-strengthening activities. The patient’s dietary compliance and the average weekly level of physical activity were recorded at each session. Diet history and levels of physical activity were collected before and after the 3-month lifestyle intervention. Food energy and nutrient intake was calculated using a computerized database and analysis program (Dietosystem version 3.0; DS Medica S.r.l., Milan, Italy). Physical activity was assessed using the short version of the International physical activity questionnaire (IPAQ) that computes the Metabolic Equivalent (MET: time spent in physical activity, expressed in minutes per week) .
At the end of the 3-month lifestyle intervention, women were encouraged to continue with the same diet and physical activity program recommended during lifestyle intervention.
The primary outcome was the proportion of women with ≥ 5 % weight loss during dietary supplements and three months after their discontinuation in GSP and placebo group.
The secondary outcomes were the changes in weight and fat mass during the intake of dietary supplements and three months after their discontinuation in GSP and placebo group.
Further outcomes were changes in blood pressure and heart rate during the intake of dietary supplements and three months after their discontinuation in GSP and placebo group.
Anthropometric measures, body composition, blood pressure (BP) and heart rate were measured at V-3, V0, V1, V2, V3, and V6. Anthropometry was measured using a body composition analyzer, which measures weight, height and Body Mass Index (BMI) with joined function of body fat analysis (Biki 300, Jawon Medical, Korea). Waist circumference was measured at the level of the umbilicus. At V0, V3 and V6, the women’s adherence to the diet was recorded by the dietician using a short questionnaire (9 items) that provided a score ranging from 0 to 18, where lower values indicate better adherence (Additional file 1). Three BP measurements separated by 5 min were obtained in sitting position at each visit and mean values were used for the analysis.
Characteristics of obese women of GSP and P group before and after the 3-month lifestyle intervention
GSP group (n = 20)
P group (n = 20)
47.6 ± 10.3
52.6 ± 9.6
94.3 ± 9.8
88.4 ± 9.1**
90.6 ± 6.6
86.3 ± 5.6**
Waist circumference, cm
112.5 ± 10.0
107.1 ± 10.1**
113.9 ± 5.4
110.3 ± 5.7**
Fat mass, kg
43.0 ± 5.8
40.9 ± 5.7**
41.9 ± 3.5
40.7 ± 3.5*
Fat mass/soft lean mass, ratio
0.98 ± 0.10
0.96 ± 0.11
1.0 ± 0.09
0.99 ± 0.08
Systolic BP, mmHg
126.0 ± 13.9
120.5 ± 10.7*
123.7 ± 10.9
119.2 ± 8.6
Diastolic BP, mmHg
79.0 ± 6.4
77.2 ± 4.7
79.5 ± 6.8
77.5 ± 5.3
Heart rate, beats/min
73.8 ± 8.6
73.1 ± 6.9
73.4 ± 9.1
70.7 ± 6.7*
The adherence to the diet progressively decreased in both groups from V0 to V6. Scores in GSP group were 5.4 ± 3.9 at V3, 2.2 ± 1.3 at V0 (p < 0.05 for V3 vs. V0), and 6.4 ± 4.9 at V6 (p = NS for V3 vs. V6). The scores in P group were 5.2 ± 4.0 at V3, 2.8 ± 1.6 at V0 (p < 0.05 for V3 vs. V0), and 7.8 ± 3.3 at V6 (p < 0.05 for V3 vs. V6). Advices on physical activity were apparently followed in both groups because fat-free mass remained stable from V0 to V6: scores in GSP group were 54.1 ± 3.3 % at V3, 53.8 ± 2.7 % at V0 (p = NS for V3 vs. V0), and 54.6 ± 3.8 % at V6 (p = NS for V0 and V3). In the P group, the scores were 52.6 ± 2.9 at V3, 53.0 ± 2.1 at V0 (p < 0.05 for V3 vs. V0) and 53.2 ± 5.1 at V6 (p < 0.05 when compared to V0 and V3).
Blood pressure and heart rate remained stable from V0 to V6 in both groups (data not shown).
Results of this study indicate that after a weight loss intervention, obese women supplemented with GSP are more likely to maintain the reduction in body weight achieved with the diet than those receiving placebo. Indeed, we observed that weight and fat mass decreased during the first month of dietary supplements with GSP and then remained stable during the period of supplement intake despite the decrease in the adherence to the diet. Conversely, the weight and fat mass of women belonging to the P group started to rise one month after the end of the lifestyle intervention. The trend to return to the pre-body fatness is an expected event after successful weight loss because of the occurrence of coordinate actions of behavioral, metabolic, neuroendocrine, and autonomic responses that are designed to maintain body energy stores . GT has been shown to stimulate daily energy expenditure by 4–4.8 % [10, 11], particularly under conditions of elevated sympathetic tone  such as obesity. GSP may be therefore more useful to offset the reduction in energy expenditure caused by the adaptive thermogenesis when given in association with a weight loss program. In fact, during a low calorie diet and adequate physical activity, the increase in the daily energy expenditure induced by GSP might not be effective enough due to the overriding effects of the initial changes in diet and physical activity. This finding is likely to explain the modest effects of GT on weight loss reported in the majority of randomized intervention studies [4, 13].
Few studies reported the effects of GT extracts on weight maintenance after a period of weight loss in overweight and moderately obese subjects. In a study by Kovasc et al., a 4-week weight loss period with a very low caloric diet was followed by a 13-week weight maintenance period in which, the subjects consumed their habitual diet and received GT-caffeine mixture or placebo. No significant differences in body weight regain were observed between the GT and placebo group however, a higher weight regain was observed in high caffeine consumers . In another study, the same group confirmed that GT–caffeine mixtures may induce further weight reductions during the weight maintenance period only in low-level caffeine consumers . These studies are not comparable to our study that utilized a GSP preparation devoid of caffeine, but with active polyphenols complexed with soy phospholipids and piperine to enhance bioavailability and stimulate thermogenesis.
We observed that the effect of Globes® on body fatness disappears after its discontinuation. This pattern does not seem be due to a lower adherence to lifestyle recommendations, because in GSP group the adherence to the diet and fat-free mass (proxy of physical activity) were similar between the end of the intake of dietary supplements and follow-up. If we consider that the decline of energy expenditure occurring after weight loss persists for at least one year , it can be assumed that GSP preparations should be taken for several months after the intensive phase of a diet to favor weight maintenance. This remains a hypothesis because energy expenditure was not assessed in this study. Future research having energy expenditure and respiratory quotient as primary endpoints would perhaps shed light on the mechanisms by which Globes® affect body fatness.
Finally, we emphasize that dietary supplements with Globes® did not affect blood pressure and heart rate levels, suggesting that the increase in sympathetic activity induced by GSP was not associated to significant cardiovascular changes.
We believe that GSP extracts devoid of caffeine may have a clinical potential for the maintenance of weight after the intentional weight loss. More prolonged randomized studies are needed to confirm these effects and evaluate the dose of GSP required to achieve expected results.
BP, blood pressure; GSP, greenselect phytosome®; GT, green tea; MET, metabolic equivalent; P, placebo; V, visit
Availability of supporting data
Reference: Dataset GSP trial in LabArchives.
LG performed the statistical analysis, interpretation of data and drafted the manuscript. CI participated in the design and coordination of the study and in the interpretation of data, and drafting the manuscript. LP recruited participants and collected data. FDP conceived the study and helped in interpreting data and revising the manuscript. All authors read and approved the final manuscript.
Francesco Di Pierro is the main formulator of Globes®. The other Authors do not report any conflict of interest.
Consent for publication
Ethic approval and consent to participate
The Ethics Committee of the Istituto Auxologico Italiano approved the study, and all subjects gave their informed consent after we provided a full explanation of the study.
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- Sumithran P, Luke BS, Prendergast A, Delbridge E, Purcell K, Shulkes A, et al. Long-Term Persistence of Hormonal Adaptations to Weight Loss. N Engl J Med. 2011;365:1597–604.View ArticlePubMedGoogle Scholar
- Rosenbaum M, Leibel RL. Adaptive thermogenesis in humans. Int J Obes. 2010;34 Suppl 1:47–55.View ArticleGoogle Scholar
- Hursel R, Westerterp-Plantenga MS. Catechin- and caffeine-rich teas for control of body weight in humans. Am J Clin Nutr. 2013;98 Suppl 6:1682–93.View ArticleGoogle Scholar
- Jurgens TM, Whelan AM, Killian L, Doucette S, Kirk S, Foy E. Green tea for weight loss and weight maintenance in overweight or obese adults. Cochrane Database Syst Rev. 2012;12:CD008650.PubMedGoogle Scholar
- Pietta P, Simonetti P, Gardana C, Brusamolino A, Morazzoni P, Bombardelli E. Relationship between rate and extent of catechin absorption and plasma antioxidant status. Biochem Mol Biol Int. 1998;46:895–903.PubMedGoogle Scholar
- Di Pierro F, Menghi AB, Barreca A, Lucarelli M, Calandrelli A. Greenselect Phytosome as an adjunct to a low-calorie diet for treatment of obesity: a clinical trial. Altern Med Rev. 2009;14:154–60.PubMedGoogle Scholar
- Belcaro G, Ledda A, Hu S, Cesarone MR, Feragalli B, Dugall M. Greenselect phytosome for borderline metabolic syndrome. Evid Based Complement Alternat Med. 2013;2013:869061.PubMedPubMed CentralGoogle Scholar
- Lambert JD, Hong J, Kim DH, Mishin VM, Yang CS. Piperine enhances the bioavailability of the tea polyphenol (−)-epigallocatechin-3-gallate in mice. J Nutr. 2004;134:1948–52.PubMedGoogle Scholar
- Craig CL, Marshall AL, Sjöström M, Bauman AE, Booth ML, Ainsworth BE, et al. International physical activity questionnaire: 12-country reliability and validity. Med Sci Sports Exerc. 2003;35:1381–95.View ArticlePubMedGoogle Scholar
- Dulloo AG, Duret C, Rohrer D, Girardier L, Mensi N, Fathi M, et al. Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans. Am J Clin Nutr. 1999;70:1040–45.PubMedGoogle Scholar
- Dulloo AG, Seydoux J, Girardier L, Chantre P, Vandermander J. Green tea and thermogenesis: interactions between catechin-polyphenols, caffeine and sympathetic activity. Int J Obes Relat Metab Disord. 2000;24:252–58.View ArticlePubMedGoogle Scholar
- Hursel R, Viechtbauer W, Dulloo AG, Tremblay A, Tappy L, Rumpler W, et al. The effects of catechin rich teas and caffeine on energy expenditure and fat oxidation: a meta-analysis. Obes Rev. 2011;12:e573–81.View ArticlePubMedGoogle Scholar
- Mielgo-Ayuso J, Barrenechea L, Alcorta P, Larrarte E, Margareto J, Labayen I. Effects of dietary supplementation with epigallocatechin-3-gallate on weight loss, energy homeostasis, cardiometabolic risk factors and liver function in obese women: randomized, double-blind, placebo-controlled trial. Br J Nutr. 2014;111:1263–71.View ArticlePubMedGoogle Scholar
- Kovacs EM, Lejeune MP, Nijs I, Westerterp-Plantenga MS. Effects of green tea on weight maintenance after body-weight loss. Br J Nutr. 2004;91:431–37.View ArticlePubMedGoogle Scholar
- Westerterp-Plantenga MS, Lejeune MP, Kovacs EM. Body weight loss and weight maintenance in relation to habitual caffeine intake and green tea supplementation. Obes Res. 2005;13:1195–204.View ArticlePubMedGoogle Scholar