Skip to main content

Table 3 Regression equations, linearity, LOD, and LOQ of the 7 marker compounds

From: Chondroprotective and anti-inflammatory effects of ChondroT, a new complex herbal medication

Compound

Linear range

(μg/mL)

Regression equationa

Coefficient of determination

LODb

(μg/mL)

LOQc

(μg/mL)

Chlorogenic acid

1.56–100.00

y = 30,420.94x – 42,881.66

0.9996

0.03

0.10

Berberine Cl

0.78–50.00

y = 81,886.13x + 16,338.31

0.9999

0.01

0.02

Nodakenin

0.78–50.00

y = 43,427.95x + 6,116.21

0.9999

0.02

0.06

Isoferulic acid

0.78–50.00

y = 44,822.70x – 7,991.61

1.0000

0.02

0.07

Oxypeucedanin hydrate

1.56–100.00

y = 26,221.17x + 5,076.06

1.0000

0.04

0.12

Decursin

0.78–50.00

y = 37,915.21x + 52,952.92

1.0000

0.04

0.12

Decursinol angelate

0.78–50.00

y = 28,967.64x + 29,015.53

1.0000

0.05

0.16

  1. ay: peak area (mAU) of compounds; x: concentration (μg/mL) of compounds
  2. bLOD = 3 × 3.3 × standard deviation of blank / slope of regression
  3. cLOQ = 10 × standard deviation of blank / slope of regression