Fig. 4

Detection of apoptotic cells through DAPI staining. a Normal cells are round in shape and show less bright blue fluorescence. Whereas the apoptotic cells are brightly stained with the characteristic features of apoptosis such as chromatin condensation, nuclear fragmentation and aggregation of apoptotic bodies etc. b Number of apoptotic cells per slide was estimated by counting apoptotic cells in five different fields. Each value represents a mean ± SD (n = 6). Significance was set at P < 0.05 (*) and P < 0.01 (**) with respect to control