Fig. 2

Oil Red O staining of HepG2 Cells. Inhibitory effect of AOE on lipid accumulation in HepG2 cell line. Red dot (arrowhead) shows lipid droplet; pale blue circle shows cell nucleus. Cells were starved for 12 h, then treated with 0.5 mM NEFA for 24 h, and then AOE for 24 h. Cells were observed under magnification of 100×, scale bar, 20 μm. AOE, Alisma orientale extract; NEFA, non-esterified fatty acid