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Table 1 Protocol of treatment of hepatocytes in synergistic studies

From: Active chemical fractions of stem bark extract of Khaya grandifoliola C.DC and Entada africana Guill. et Perr. synergistically protect primary rat hepatocytes against paracetamol-induced damage

Groups

Volume of cell suspensions (μl)

Volume of fractions (μl)

Volume of Toxicant or vehicle (μl)

Plant fraction/combination concentration tested

I

980

10

10

10 EC50 EaF10

II

980

10

10

10 EC50 KgF25

III

980

10

10

10 EC50 EaF10 + 1 EC50 KgF25

IV

980

10

10

10 EC50 EaF10 + 3 EC50 KgF25

V

980

10

10

10 EC50 EaF10 + 5 EC50 KgF25

VI

980

10

10

10 EC50 KgF25 + 1 EC50 EaF10

VII

980

10

10

10 EC50 KgF25 + 3 EC50 EaF10

VIII

980

10

10

10 EC50 KgF25 + 5 EC50 EaF10

IX

980

10

10

10 EC50 KgF25 + 10 EC50 EaF10

silymarin

980

10

10

10 EC50 silymarin

paracetamol

990

0

10

0

control

990

0

10

0

  1. EC50: half efficient concentration; KgF25: methylene chloride/methanol (75:25 v/v) fraction of K. grandifoliola; EaF10: methylene chloride/methanol (90:10 v/v) fraction of E. africana. EC50 values are: 13.47 ± 2.06, 10.30 ± 1.66 and 13.71 ± 3.87 μg/ml for EaF10, KgF25 and silymarin, respectively. 10 EC50 EaF10, 10 EC50 KgF25 and 10 EC50 sil mean the fractions EaF10, KgF25 and silymarin have been tested at the final concentration of 130 μg/ml (10 × 13 μg/ml = 130 μg/ml), 100 μg/ml (10 × 10 μg/ml = 100 μg/ml) and 130 μg/ml (10 × 13 μg/ml = 130 μg/ml), respectively