Fig. 5
Effect of PTS on the phosphorylation of ERK2 (a) and p38 (b) in the agonists-activated platelets. Washed platelets (4 × 108 cells · ml-1) were pre-incubatted with vehicle or PTS at the concentrations indicated prior to stimulation with thrombin (0.1 U/ml) (A and B), collagen (2.5 μg · ml-1) (c and d) or ADP (10 μM) (e and f). Proteins were extracted, separated by SDS-PAGE transferred to PVDF and immunoblotted with antibodies against p-ERK, p-p38 and β-actin. Blots were visualized by ECL. The blots were representative of three independent experiments. The value was expressed as each normalized data relative to agonist alone