Fig. 5From: Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized ratsEffects on the expression of estrogenic activities. Transcription of ERα and ERβ activated by the mixture of BDD in Hela cells. Hela cells were transiently cotransfected with pERE-luc, pRL-TK, and ERα or ERβ by lipofection. The cells received no treatment, treatment with 10 nM E2, or treatment with varying mass concentrations of each of the mixture alone. Estrogen-stimulated luciferase activity of ERs was measured and the data was presented as the fold-increase over control + standard deviation, and fold-increase representative of three experiments done in triplicate. ** P < 0.01 vs. control; ◆◆ P < 0.01 vs.E2 Back to article page