Fig. 2
Effects of RCE on glycogen synthesis in HepG2 cells. HepG2 cells were cultured in 24-well plates and exposed to medium without serum for 24 h. The cells were then incubated with RCE (μg/mL) for 6 h or insulin (Ins, 100 nM) for 1 h before 14C (U)-d-glucose was added. The intensity of 14C in glycogen was analyzed (a). Expression levels of p-GSK3ß and p-GS were analyzed by western blotting (b). A quantitative analysis of the relative levels of p-GSK3ß (c) and p-GS (d) was conducted. The results represent the mean ± SEM (n = 3), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. the sample without RCE