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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: All-trans retinoic acids induce differentiation and sensitize a radioresistant breast cancer cells to chemotherapy

Fig. 6

ATRA exposure increases the sensitivities of MCF7/C6 cells to chemotherapeutic treatments. a Effect of ATRA on clonogenic survival and cell cycle distribution in epirubincin-treated MCF7/C6 cells. Cells were pretreated with 1.0 μM ATRA, or DMSO as control, for 72 h, and 500 cells were then plated and treated with indicated concentrations of Epirubicin. 24 h later, cells were washed with fresh medium and were then maintained for colony formation assay, or collected for cell cycle analysis. Top left: Survival curve for colony formation; Top right: demonstrative images for colony survival of epirubincin-treated cells. Bottom: ATRA-induced cell cycle changes in epirubincin-treated cells. b Effect of ATRA on responses of MCF7/C6 cells to treatments of 5-Fu and Doxetaxel. Cells were pretreated with ATRA as described above, and were then treated with 1.0 μg/mL of 5-Fu or 0.5 nM of Doxetaxel for 24 h. Colony formation experiments were then performed. Top left: Diagram showing the change of colony formation in cells exposed to 5-Fu treatment; top right: demonstrative images for colony survival of 5-Fu-treated cells; Bottom left: Diagram showing the change of colony formation in cells exposed to Doxetaxel treatment; Bottom right: demonstrative images for colony survival of 5-Fu-treated cells. Data represent the average from at least three independent experiments. *Indicates statistical significance (p < 0.05)

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