Fig. 7
γH2AX-based DNA damage in CPC-treated oral cancer Ca9-22 cells and the effect of NAC pretreatment. With or without 2 mM NAC pretreatment for 1 h, cells were treated with 0, 3, 6, 9, and 12 μM of CPC for 24 h. a Representative flow cytometry-based DSB profile for CPC-treated Ca9-22 cells. Dashed lines indicate the γH2AX positive regions in each panel. b Quantification analysis of fold changes in γH2AX-based DNA damage in CPC-treated Ca9-22 cells. Data, mean ± SD (n = 3). For the same drug treatment of different concentrations, data marks (a to e) without overlapping by the same lower-case letter significantly differed (one-way ANOVA with Tukey HSD Post Hoc Test)