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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Clarification of the molecular pathway of Taiwan local pomegranate fruit juice underlying the inhibition of urinary bladder urothelial carcinoma cell by proteomics strategy

Fig. 3

Confirmation of the impacts of PEE on the expression levels of diablo, profilin 1, TPI1, NADUFAF1, PI31 in T24 cell by western immunoblotting. a Diablo. b Profilin 1. c TPI1. d NADUFAF1. e PI31. Human T24 cells were incubated with 50 μg/mL PEE for various durations (hours). Western immunoblotting was carried out as described in section 2. The blot was the typical data of three independent studies. The protein expression fold (mean ± S.D.) was expressed as the ratio of normalized intensity (observed protein/actin or GAPDH) at each time point divided by that at the beginning of treatment (0 h). * and ** represented P < 0.05 and P < 0.001 respectively as compared to untreated cell using Student’s t-test

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