Rutin attenuates intestinal toxicity induced by Methotrexate linked with anti-oxidative and anti-inflammatory effects

Table 3 Effects of rutin on intestinal cytokines induced by methotrexate in rats

Groups	Treatment	IL-2 (pg/ml)	IL-4 (pg/ml)	IL-10 (pg/ml)
Group-I	Sham control (Normal saline, 3.0 ml/kg)	398.10 ± 5.32	162.18 ± 8.79	63.09 ± 5.51
Group-II	Toxic control (MTX, 2.5 mg/kg)	671.93 ± 56.65^a	213.79 ± 7.64^a	88.57 ± 3.45^a
Group-III	Rutin (50 mg/kg)	174.15 ± 8.20^***a	193.50 ± 1.47^***a	104.05 ± 0.65^**a
Group-IV	Rutin + MTX(50 + 2.5 mg/kg)	233.52 ± 14.69^***a	117.29 ± 0.023^***a	63.21 ± 2.96
Group-V	Rutin (100 mg/kg)	178.72 ± 3.28^***a	198.26 ± 7.76^***a	122.11 ± 1.88^***a
Group-VI	Rutin + MTX (100 + 2.5 mg/kg)	272.02 ± 15.00^***a	179.24 ± 1.57^***	96.99 ± 1.50^***a

Each group contains six animals. Values are represented as mean ± SEM
Statistical significance compared to toxic control using one-way ANOVA followed by Bonferroni test
*P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant
Statistical significance compared to normal control (Group-I) using one-way ANOVA followed by Bonferroni test
^c P < 0.05, ^b P < 0.01 and ^a P < 0.001 were considered statistically significant

ISSN: 2662-7671