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Table 2 Effects of rutin on intestinal oxidative damage induced by methotrexate in rats

From: Rutin attenuates intestinal toxicity induced by Methotrexate linked with anti-oxidative and anti-inflammatory effects

Groups Treatment TBARS (nM of MDA/mg of protein) GSH (mg%, 1 × 10−4) SOD (unit of SOD/mg of protein) Catalase (nM at H2O2/min/mg of protein) Protein carbonyl (nM/ml)
Group-I Sham control (Normal saline, 3.0 ml/kg) 0.82 ± 0.18 30.63 ± 2.38 1.82 ± 0.035 1.19 ± 0.35 38.41 ± 1.61
Group-II Toxic control (MTX, 2.5 mg/kg) 5.57 ± 0.12a 117.17 ± 1.12a 0.29 ± 0.035a 0.45 ± 0.03*** 42.38 ± 0.35
Group-III Rutin (50 mg/kg) 5.05 ± 0.47***a 63.65 ± 1.20***a 1.31 ± 0.023***a 1.50 ± 0.54 16.36 ± 0.23***a
Group-IV Rutin + MTX (50 + 2.5 mg/kg) 4.49 ± 0.25***a 89.48 ± 1.13***a 1.58 ± 0.005***a 0.40 ± 0.07** 27.27 ± 0.91*b
Group-V Rutin (100 mg/kg) 2.87 ± 0.13***a 48.76 ± 1.06***a 1.55 ± 0.011***a 0.78 ± 0.33 24.09 ± 0.46**a
Group-VI Rutin + MTX (100 + 2.5 mg/kg) 2.97 ± 0.21***a 51.92 ± 1.70***a 2.63 ± 0.046***a 1.66 ± 0.48* 31.52 ± 5.76
  1. Each group contains six animals. Values are represented as mean ± SEM
  2. Statistical significance compared to toxic control using one-way ANOVA followed by Bonferroni test
  3. *P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant
  4. Statistical significance compared to sham control (Group-I) using one-way ANOVA followed by Bonferroni test
  5. c P < 0.05, b P < 0.01 and a P < 0.001 were considered statistically significant