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Antidiabetic potential of two medicinal plants used in Gabonese folk medicine
© Agnaniet et al. 2016
Received: 18 June 2015
Accepted: 16 February 2016
Published: 22 February 2016
Diabetes mellitus is a metabolic disorder which is rising globally in rich and developing countries. In the African region this rate is the highest, with 20 million diagnosed diabetics. Despite a noticeable progress in the treatment of diabetes mellitus by synthetic drugs, the search for new natural anti-diabetic agents is going on. Nauclea diderrichii (De Wild.) Merr. (ND) and Sarcocephalus pobeguinii Hua ex Pellegr. (SP) are used as traditional medicines in Gabon for the treatment of different diseases, especially in the case of diabetes. The aim of this study was to evaluate the antidiabetic potential of these two medicinal plants traditionally used in Gabon.
Pharmacological (inhibitory action on α and β-glucosidases) and toxicological (effect on human T cell proliferation) studies were conducted on aqueous extracts of ND (leaves and bark) and SP (bark) collected in Gabon. All raw extracts were analyzed by HPTLC and their content in phenolic compounds was determined by using standard method. The most active extracts were submitted to preparative HPLC in order to evidence the most efficient subfractions by biological evaluation.
The results showed that two extracts from ND were potent α-glucosidase inhibitors, the leaf extract being more active that the bark extract: the first one was more than 60 fold more active than Acarbose, which is an oral medication used to treat type 2 diabetes; the extract from SP bark was less efficient. The HPLC subfractions of the extracts of ND leaves and SP bark were tested in the same experimental conditions. In each case, the most active subfractions still show very potent inhibitory effect on α-glucosidase (80-90 % inhibition at 0.1 mg/mL). The most efficient extract, from ND leaves, was also characterized by the highest percentage of phenolic compounds, which suggests a relationship between its inhibitory potential on α-glucosidase and its content in phenolic compounds. Conversely, only a moderate inhibitory activity of the three extracts was observed on β-glucosidase.
These results clearly indicated that active compounds present in N. diderrichii and S. pobeguinii leaves or/and bark were selective and highly potent inhibitors of α-glucosidase and validate their popular use for the treatment of diabetes.
Diabetes mellitus is a metabolic disorder, mainly of two types (1 and 2) and characterized by chronic hyperglycemia with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action or both [1–3]. Currently, the number of diabetics sharply increases in rich and developing countries. In 2013, the overall prevalence of diabetes in the world’s population was estimated to 8.3 % approximately . In the African region, and particularly in Sub-Saharan Africa, this rate is the highest, with 20 million diagnosed diabetics .
Oral hypoglycemic agents, such as α-glucosidase inhibitors, exert their effects via a variety of mechanisms, which include the reduction of hepatic glucose production, the enhancement of insulin secretion by pancreatic β-cells and the improvement of insulin sensitivity. Glucosidase inhibitors have been the subject of extensive interest  because of their potential as drugs for the treatment of diabetes . Alpha-glucosidase is a membrane-bound enzyme, at the epithelium of the small intestine; it hydrolyses the cleavage of glucose from disaccharides and oligosaccharides. The inhibitors of this enzyme delay carbohydrate hydrolysis, prolong the overall carbohydrate digestion time and thus cause a reduction in glycaemia . Therefore, inhibition of α-glucosidase is considered as an important way to treat non-insulin-dependent diabetes. This hypothesis was successfully confirmed in animal and clinical studies by the administration of various α-glucosidase inhibitors [9, 10].
Despite considerable progress in the treatment of diabetes mellitus by synthetic drugs, the search for new natural anti-diabetic agents is going on. The world health organization (WHO) estimates that 80 % of the population in some developing countries, especially in Sub-Saharan Africa, still use the traditional medicine . Several plants are used in folk medicine for their hypoglycemic activity and they were investigated using different experimental methods . Thanks to these studies, natural glycosidase inhibitors have been discovered and tested for their activity [13, 14].
Gabon has remarkable biodiversity and rich cultural traditions of plant use. Scientific understanding of medicinal plants is however largely unexplored and pharmacological investigation of Gabonese flora only gained momentum recently. Rubiaceae are among plants of wide usage in traditional medicine in the Sub-Saharan region  more particularly Nauclea and Sarcocephalus species, which belong to the tribe Naucleae, subtribe Naucleinae . In Gabon, the species Nauclea diderrichii and Sarcocephalus pobeguinii (syn. Nauclea pobeguinii) are used in folk medicine for the treatment of different diseases, especially in the case of diabetes associated or not to hypertension. In the Eviya area, S. pobeguinii is also used in leaf infusions as febrifuge, while bark maceration is indicated for urogenital infections . The traditional use of both species is also mentioned in ethnomedicinal reports from other African countries: N. diderrichii is used in Nigeria against arthritis and malaria [18, 19] while S. pobeguinii was reported for several indications in Cameroon , Nigeria [21, 22], Guinea  and Senegal .
N. diderrichii is an evergreen tree that reaches a height of 30-40 m and a diameter of 0.9-1.5 m; its natural habitat is subtropical or tropical moist lowland forests; the wood of this tree, which is known as bilinga, is dense and resistant to fungi and insects . S. pobeguinii is a forest tree or shrubby tree 6–30 m tall; its bark is whitish, grey or pale brown, roughly fissured, flaking in papery scales about 2 cm in diameter; its fruits are edible .
The chemical investigations on these two species are numerous: they concern alkaloids from N. pobeguinii [27, 28] whereas alkaloids [29–31], saponins [32, 33] and other chemical classes [34, 35] were obtained from different plant parts of N. diderrichii. A few biological studies have been conducted on these species. Water or/and ether extracts of N. diderrichii from Gabon were evaluated for their anti-malarial  and anti-leshmanial  activities. The same species collected in the West African region was screened for its antiplasmodial activity .
Nevertheless, to the best of our knowledge, N.diderrichii and S. pobeguinii were not screened for their hypoglycemic activity despite their traditional use for diabetes. A literature survey shows only some studies in this field on plants belonging to the same genera, such as Sarcocephalus latifolius from the Central Region of Togo  and Nigeria  or Nauclea latifolia collected in Benin (roots and stem)  or in Nigeria (leaves) . This led us to carry out phytochemical and biological investigations on the two species collected in Gabon: bark and leaf extracts of N. diderrichii as well as bark extract of S. pobeguinii.
Plant extracts were prepared and tested in human cells to determine their capacity to inhibit α- and β-glucosidases, to investigate their biocompatibility with cells and to provide pharmacological basis for their traditional use as anti-diabetic agent.
The plant selection was based on their use by local population and traditional healers. Thirty people were interviewed in 10 villages located in the Nkomo Mondah department (Province de l’Estuaire), in the Ogooué as well as in the Lakes departments (Province du Moyen Ogooué). These data were supplemented by literature information [17, 23, 47–49]. The plants were collected during the short dry season: N. diderrichii was collected in Libreville in December 2009 (department of Nkomo-Mondah) and S. pobeguinii was collected in Lambarene in January 2010 (department of Ogooue). They were identified by Y. Issembe and R. Niangadouma, botanists at the National Herbarium of the Institute of Pharmacopea and Traditional Medicine (IPHAMETRA) where voucher specimens were deposited under the following numbers: Nauclea diderrichii (De Wild.) Merr. [Wilks: 8836 (WAG), 1988; 2438 (LBV, WAG), 1991] and Sarcocephalus pobeguinii Hua ex Pellegr. [Azizet Issembé 172 (LBV, WAG), 2000; Wilks: 1035 (LBV, WAG)]. Leaves and bark of N. diderrichii as well as bark pieces of S. pobeguinii were collected and air dried in a room maintained at a controlled temperature (25 °C) using an air-conditioning unit; then, the samples were stored in a dry place until use.
Preparation of plants extracts
In the case of N. diderrichii, two parts of the plant were extracted: raw leaves and ground bark. Plant material was macerated in water (100 g of plant sample in 1 L of water at room temperature). After filtration on büchner (paper Whatman N°3, medium porosity, particle retention 6 μm, diam. 9 cm), the filtrates were lyophilized to give the samples E 14 (leaves) and E 15 (bark) with 13.9 % yield and 15.0 % yield respectively. The ground bark of S. pobeguinii was extracted by decoction. Briefly, 100 g of plant material was boiled in 1 L of water for 30 min. After filtration on paper Whatman N°3 (medium porosity, particle retention 6 μm, diam. 9 cm), the filtrate was cooled at room temperature and lyophilized to obtain 10.34 g of the extract E 10.
Phytochemical analysis by HPTLC
Phytochemical analysis of the extracts was undertaken by HPTLC method using HPTLC plate silica gel F254s (Merck) 20×10 cm and different derivatizations. All reagents and solvents were of analytic grade.
The samples were tested following the CAMAG Standard Operating Procedures (SOP): 200 mg of dry extracts were dissolved in 10 mL of ethanol:water 1:1, sonicated twice during 10 min and centrifuged. The supernatant was used for the analyses; applications volumes: 2 and/or 5 μL for samples and standard solutions with Automated TLC Sampler 4, band length 8 mm, distance from lower edge 8 mm, distance from left and right edge 20 mm, track distance min. 10 mm; development in the Automatic Developing Chamber ADC2 with chamber saturation during 20 min and with adjustment of relative humidity to 33%rH. The migration distance (from lower edge of plate) was 70 mm; different mobile phases and derivatization reagents were used: S1 (toluene : ethyl acetate 19:1), S2 (chloroform : methanol : water 35:15:2), S3 (ethyl acetate : acetic acid : formic acid : water100:11:11:27), S4 (1-butanol : acetic acid : water 7:1:2) and S5 (chloroform : methanol : water 70:30:4).
The most suitable solvent system combined with the adequate derivatization was selected for the performance: the best resolution was most often observed with mobile phases S2 and S3 for N. diderrichii and mobile phase S2 in the case of S. pobeguinii.
Anisaldehyde sulphuric acid reagent (AAS) (for mobile phases S1, S2 and S4) for saponins detection under white light
NP/PEG reagent (S3) for flavonoids detection under 366 nm
Dragendorff reagent (S5) for alkaloids detection under white light
2,2-Diphenyl-picrylhydrazyl reagent (DPPH) for screening of antioxidant properties of substances (S3).
Observation was performed using TLC Visualizer under UV 254 and 366 nm prior to derivatization and under UV 366 nm and white light illumination (remission and transmission mode) after derivatization.
Determination of total phenolic compound content
Folin-Ciocalteu reagent F 9252 lot # BCBF 2476 was purchased from Sigma-Aldrich Co (France). Total phenols content was performed by the Folin-Ciocalteu (FC) colorimetry method (FC)  which is based on a chemical reduction of the reagent, a mixture of tungsten and molybdenum oxides. Although not very specific for phenolics, the protocol gives a good idea of the total phenols content. Gallic acid was employed as calibration standard and results were expressed as gallic acid equivalents. Briefly, 0.5 mL of gallic acid solution (10-100 mg/L) was mixed with 0.25 mL FC reagent (1 N). After five minutes, 1.25 mL sodium carbonate (20 % w/v) was added. The mixture was shaken and left during one hour at room temperature. Absorbance A was measured at 725 nm with different concentrations (c) of gallic acid. The linear regression A = f(c) was carried out with Microsoft Excel. The same protocol was used with the plants extracts (0.2-1 g/L) to evaluate their phenols content. The results were expressed in mg of gallic acid equivalents (GAE) per g extract. All experiments were repeated three times. The results are expressed as the mean ± standard deviation (SD).
Fractionation by HPLC
The HPLC purifications were run on a Waters 4000 preparative apparatus on a C18 Deltapak column (100 mm × 40 mm, 15 μm, 100 Å), with UV detection at 214 nm, at a flow rate of 50 mL/min of a mixture of A (water with 0.1 % TFA) and B (acetonitrile with 0.1 % TFA) in a gradient mode. E 10 was purified starting from 100 % A to 100 % B in 20 min after an isocratic run of 5 min in 100 % A. E 14 was purified in a gradient mode from 100 % A to 50 % B in 25 min. The samples were collected manually every two minutes and the fractions were lyophilized.
α-Glucosidase and β-glucosidase inhibitory assays
Acarbose, α-glucosidase from Saccharomyces cerevisiae and β-glucosidase from almond were obtained from Sigma, isofagomine was synthesized as previously reported . Typical procedure for inhibition studies: α- and β-glucosidase activities were assayed with either 8 mM solution of 4-methyl umbelliferyl α-D-glucopyranoside or 4-methyl-umbelliferyl β-D-glucopyranoside (Carbosynth) in 100 mM NaHPO4 buffer, pH 6.8 at 30 °C in a 384-well microplate. 10 mM Acarbose and 1 μM isofagomine were used as reference inhibitors for α- and β-glucosidases, respectively. Fluorescence was monitored (λexcitation = 364 nm, λemission = 445 nm) over a 20 min period, in the presence of the extract/compound solutions in DMSO compared to the same volume of DMSO alone. The fluorescence increase reflected the cleavage of umbelliferyl glucopyranosides: the value 100 % was attributed to the fluorescence obtained with enzymes in the presence of DMSO alone. In incubations with extracts or compounds, remaining activity (RA, expressed as a percentage) was calculated as the ratio [fluorescence released in the presence of extracts/compounds]/[fluorescence released in the presence of DMSO alone] × 100 and shown in Figs. 2, 3, 4 and 5 for every concentration of extract/compound. Finally, the percentage inhibition was calculated from these values following the equation: 100-RA.
For the initial screening, extracts dissolved in DMSO were added at a final concentration of 1 mg/mL. Active extracts and separated fractions were diluted in DMSO and added at concentrations ranging 0.01 to 1 mg/mL in duplicate.
Cell culture and cytotoxicity assay
Human breast (MCF-7) cancer cells were purchased from ATCC (American Type Culture Collection, Manassas, VA). MCF-7 cells were cultured in DMEM-F12 culture medium supplemented with 10 % foetal bovine serum and gentamycin 50 μg/mL. These cells were allowed to grow in humidified atmosphere at 37 °C under 5 % CO2. For cytotoxicity analysis, MCF-7 cells were seeded into 96-well plates at 2.104 cells per well in 200 μL culture medium and allowed to grow for 24 h. Then cells were treated for 48 h with or without extracts at 0.5 and 1 mg/mL. After incubation, a MTT assay was performed to evaluate the cytotoxicity . The assay detects the reduction of MTT [3-(4,5-dimethylthiazolyl)-2,5-diphenyl-tetrazolium bromide] by mitochondrial dehydrogenase to blue formazan products, which reflects the normal functioning of mitochondrial and cell viability . Briefly, 0.5 mg/mL MTT reagent (20 μL) was added to each well and incubated for additional 4 h. Then, the medium was removed and 150 μL of EtOH/DMSO (1:1) was added to MTT precipitates in each well to solubilize the formazan crystals. The plates were read for optical density at 540 nm, using microplates reader (Multiskan).
Results and discussion
Of all the collected information, it appears firstly that populations refer to the experiences of others to use medicinal plants as a remedy against well-defined diseases in accordance with a transmission of traditional practices from one generation to another, or refer to traditional healers. Usually, the dose remains uncertain. In addition, the traditional healers do not generally identify stricto sensu the diabetic or hypertensive pathology, but they treat the characteristic symptoms associated with them. Among all the data, S. pobeguinii and N. diderrichii were the most cited for the various therapeutic applications mentioned above, and ethnobotanical survey reported the use of macerate or decoction of bark of both species to cure diabetes as well as hypertension. Finally, these species were harvested in their natural habitat.
E 10 = 84.5 mg ± 1.5 GAE/g extract
E 14 = 148 mg ± 3 GAE/g extract
E 15 = 75.2 mg ± 2.6 GAE/g extract
Extract 14, obtained from N. diderrichii leaves, is characterized by the highest percentage of phenolic components while both extracts from bark of N. diderrichii (E 15) and S. pobeguinii (E 10) contained lower amounts of compounds belonging to this chemical class. These results are corroborated by the HPTLC analysis using DPPH reagent which shows some nice defined white zones for the three extracts, mainly in the case of E14, indicating a high radical scavenging effect, mainly located in the polar fraction (see Additional file 1).
Phytochemical test results by HPTLC of S. pobeguinii and N. diderrichii extracts
Radical scavenging activity
S. pobeguinii bark(E10)
N. diderrichii leaf (E14)
N. diderrichii bark (E15)
Preliminary screening of raw extracts on α-glucosidase activity
Nevertheless, it is noteworthy that the extracts from N. diderrichii (E 14 and E 15) displayed a higher inhibitory potency than the reference compound acarbose, which is an oral medication used to treat type 2 diabetes. Indeed, E14, which induced 100 % inhibition at 0.1 mg/mL, was more than 60 fold more active than Acarbose (63 % inhibition at 10 mM final concentration = 6.45 mg/mL).
The high activity observed for the extract obtained from of N. diderrichii leaves (E 14), which is the richest in phenolic components, suggests a direct relationship between its inhibitory potential of α-glucosidase and its content in phenolic compounds.
Selective inhibition by raw extracts
Fractionation of extracts and screening on α-glucosidase activity
The present study clearly indicates that compounds present in extracts of N. diderrichii (E 14 and E 15) and S. pobeguinii (E 10) are potent and selective inhibitors of α-glucosidase. A comparison with the results obtained with acarbose, which is used as oral medication to treat type 2 diabetes, indicates that the most active extract, obtained from N. diderrichii leaves, is more than 60-fold more active than the commercial antidiabetic drug. In addition, at the concentration inducing the highest effect on α-glucosidase inhibition, no toxicity was observed on human cell line. This demonstrates the safety of these extracts and permits to allow their use.
Recently, extracts with high phenolic contents have shown a strong capacity to inhibit α-glucosidase activity [54–56] and to treat diabetic rats . The same correlation is observed in our study, the most active extract (E14), obtained from N. diderrichii leaves, being also the richest in phenols. Therefore, we can postulate that the antidiabetic activity of N. diderrichii and S. pobeguinii extracts might be related to their phenolic compounds content, even if we cannot exclude the participation of other classes of compounds.
Finally, our results validate the folk medicinal use of S. pobeguinii and N. diderrichii for their hypoglycemiant effect. Since these plants are widely available in Gabon, they could be a source of cheap and effective medication for persons with high blood glucose levels.
We are mostly grateful to Mr Y. Issembe and Mr R. Niangadouma, from the National Herbarium of the Institute of Pharmacopea and Traditional Medicine (IPHAMETRA) for their assistance with the collection and identification of plant materials.
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