Table 1 Preliminary qualitative phytochemical analysis of Passiflora incarnata methanolic extract (PI-ME)
Sample | Test | Observation | Result |
|---|---|---|---|
1. | Aqueous solution of PI-ME + 10 % ammonium hydroxide solution | Appearance of yellow coloration | Flavonoids present |
2. | A portion of PI-ME + few drops of Wagner’s reagent | Reddish brown precipitate | Alkaloids present |
3. | A small volume of PI-ME + 1–2 drops of Mayer’s reagent | Creamy or white precipitate | Alkaloids present |
4. | 0.5 ml PI-ME + 0.5 ml benedict’s reagent → mixed and boiled for 2 min | Characteristic colored precipitate | Carbohydrates present |
5. | 1 ml PI-ME + 1 ml Barfoed’s reagent → boiled for 2 min | Red precipitate | Carbohydrates present |
6. | 50 mg PI-ME + 5 ml distilled water + small amount of 5 % ferric chloride solution | Intense green coloration | Tannins and phenolic compounds present |
7. | 50 mg PI-ME + conc. HCL → heated on water bath for 2 h → resultant hydrolysate filtered → 2 ml hydrolysate + 3 ml chloroform → chloroform layer separated out + 10 % ammonia solution | Pink coloration | Glycosides present |
8. | A small amount of PI-ME → compressed between two pieces of filter paper | Formation of oil spot on filter paper | Fixed oils present |
9. | 50 mg PI-ME + 20 ml distilled water → shaken for 15 min | Formation of 2 cm thick layer of foam | Saponins present |