Fig. 3From: Electroacupuncture prevents white adipose tissue inflammation through modulation of hypoxia-inducible factors-1α-dependent pathway in obese miceEffect of electroacupuncture on adipose tissue macrophage cell marker (F4/80) and inflammation mRNA levels of chemokines in ob/ob mice. Histomorphometrical analysis of adipose tissue sections stained with F4/80 (original magnification 100) (a) and mRNA quantitated in mice adipose tissue by quantitative reverse transcription polymerase chain reaction (b). Inhibitory effects of EA on the NF-kB-involved pathway in the adipose tissue. Nuclear protein extracts and total cell protein of the white adipose tissue were prepared and measured by Western blot analysis (c). Quantification of the protein expression of p65 in the nucleus and cytoplasm shown as the relative intensity of the protein bands. Blot showing the inhibition of the phosphorylation of the inhibitor of IκBα by EA. Each result represented the experiment performed in triplicate assays in the different experimental groups. Arrows indicate F4/80 positive cells. Results are the means ± S.E.M. *P < 0.05, compared with C57BL/6 J mice, #P < 0.05, compared with ob/ob mice. EA, electroacupuncture. 3 T, three times EA treatment. 7 T, seven times EA treatmentBack to article page