Fig. 5

Suppression of LPS-induced AP-1 activation by HHS in RAW 264.7 macrophages. a The cells were treated with 300 μg/ml of HHS for 1 h before LPS treatment (100 ng/ml) for 30 min. Nuclear proteins were resolved on 10 % SDS-polyacrylamide gels, followed by Western blotting using the indicated antibodies. Lamin B was used as an internal control for the nuclear fractions. b In a parallel experiment, nuclear extracts were prepared, and the DNA binding activity of AP-1 was analyzed by an EMSA