Fig. 4

Effect of butein on PI3K/Akt/mTOR, ERK and FAK pathways. a B16F10 cells were incubated in the absence or presence of butein (1- to 10 μM) for 12 hrs. Then, the cells were harvested and lysed for the detection of the PI3K/Akt/mTOR, ERK and FAK pathways activation by Western blot. The quantitative densitometry of the relative level of protein phosphorylation (phosphortlation protein/total protein) was performed with Image-Pro Plus and was shown in b. Data are expressed as mean ± S.E.M. of five independent experiments. The protein phosphorylation in all treatment cells were significant lower than control (Veh) group (***p < 0.001 as compare with control (Veh) group)