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Table 2 Summary of reaction conditions to determine modes of CYP enzyme inhibition and Ki values

From: Effect of an ethanol extract of Descurainia sophia seeds on Phase I and II drug metabolizing enzymes and P-glycoprotein activity in vitro

Parameters

CYP1A2

CYP2C9

CYP2C19

Substrate (μM)

Luciferin-ME

Luciferin-H

Luciferin-H EGE

 

(12.5 ~ 100)

(25 ~ 200)

(2.5 ~ 20)

Enzyme (pmol)

0.5

0.5

0.25

EEDS (μg/mL)

0-100

0-100

0-100

Incubation time (min)

10

30

20

Temperature (°C)

37

37

37

  1. Luciferin-ME luciferin 6-methyl ether, Luciferin-H 6-deoxyluciferin, Luciferin-H EGE ethylene glycol ester of 6-deoxyluciferin