Effect of an ethanol extract of Descurainia sophia seeds on Phase I and II drug metabolizing enzymes and P-glycoprotein activity in vitro

Table 1 Summary of assay systems for human Phase I/II DME and P-gp inhibition assays

Types	DMEs	Assay kits		Microsomes		Specific inhibitors
Types	DMEs	Suppliers	Cat #	Suppliers	Cat #	Chemicals	Suppliers	Codes
Ph I	CYP1A2	Promega	V8772	Invitrogen	P2792	α-naphthoflavone	Sigma-Aldrich	N5757
	CYP2C8	Promega	V8782	Invitrogen	PV6138	Montelukast	Cayman	10008318
	CYP2C9	Promega	V8792	Invitrogen	P2378	Sulfaphenazole	Sigma-Aldrich	S0758
	CYP2C19	Promega	V8882	Invitrogen	P2864	Ticlopidine	Sigma-Aldrich	T6654
	CYP2D6	Promega	V8892	Invitrogen	P2283	Quinidine	Sigma-Aldrich	Q3625
	CYP3A4	Promega	V8802	Invitrogen	P2858	Ketoconazole	Sigma-Aldrich	K1003
Ph II	UGT1A1	Promega	V2120	Microsome supplied in assay kit		Diclofenac	Sigma-Aldrich	D6899
	UGT2B7	Promega	V2130	Microsome supplied in assay kit		Diclofenac	Sigma-Aldrich	D6899
Drug transport	P-gp	Promega	V3601	Microsome supplied in assay kit		Na₃VO₄ supplied in assay kit

ISSN: 2662-7671