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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: In vitro antiviral activity against rotavirus and astrovirus infection exerted by substances obtained from Achyrocline bogotensis (Kunth) DC. (Compositae)

Fig. 2

Evaluation of the virucidal effect exerted by F1 fraction from Achyrocline bogotensis (Kunth) DC. (Compositae) on (a) rotavirus (RRV strain/MOI = 5) and (b) astrovirus (Yuc8 strain/MOI = 2). Viruses were first co-incubated with several nontoxic concentrations of F1 during 1 h at 37 °C, and then mixtures were placed on Ma104 and Caco2 cells, respectively. At 10 or 12 hpi intracellular virions were immunodetected in order to find a decrease of the infectivity. Viruses co-incubated with the corresponding concentrations of the solvent of F1 (DMSO), were used as controls. The pictures were taken with a 10X objective in an Olympus CKX41 microscope

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