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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: The effect of 3β, 6β, 16β-trihydroxylup-20(29)-ene lupane compound isolated from Combretum leprosum Mart. on peripheral blood mononuclear cells

Fig. 4

Effect of lupane on topoisomerases I and II activity. Electrophoresis in 1 % agarose gel to assess the activity of TOPO I (a): nuclear extracts of PBMCs and supercoiled DNA (TopoGen) (control), lupane (6; 1.5 or 0.7 μg/mL plus nuclear extract of PBMCs and supercoiled DNA; markers (relaxed and supercoiled DNA). (Representatives of 2n). Electrophoresis in 1 % agarose gel with ethidium bromide to assess the activity of TOPO II. b: nuclear extract of PBMCs and kDNA (TopoGen) (control), lupane (6; 1.5 or 0.7 μg/mL plus nuclear extract of PBMCs plus kDNA, nuclear extract of PBMCs plus kDNA plus etoposide (inhibitor); markers (linear and decatenated kDNA). (Representatives of 2n)

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BMC Complementary Medicine and Therapies

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