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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: The effect of 3β, 6β, 16β-trihydroxylup-20(29)-ene lupane compound isolated from Combretum leprosum Mart. on peripheral blood mononuclear cells

Fig. 3

Effect of lupane on the release of TNF-α (a) and IL-10 (b) by PBMCs. PBMCs (2×105) were incubated with lupane at 0.3, 0.7 and 1.5 μg/mL or RPMI (negative control) or LPS (positive control, 1 μg/mL) at 37 °C in a humidified atmosphere of 5 % CO2 for 15 h. The concentrations of TNF-α and IL-10 in the supernatant were quantified by a specific Enzyme Immune Assay (EIA). The results were expressed in pg/mL of TNF-α or IL-10 and represent the mean ± S.E.M. of four donors. ***P < 0.001 compared to control (ANOVA)

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