Fig. 4From: Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca2+ and ERK1/ERK2 signalingCa2+ signaling modulation by PGL and PGPC in splenic T-cells. Cells (2 × 106/ml) were loaded with the fluorescent probe, Fura-2/AM, as described in Methods. The arrow head indicates the time when PGL or PGPC were added into the cuvette without interruptions in recordings. Figures show the single traces of observations which were reproduced independently (n = 6). Inserts show the increase in [Ca2+]i evoked by the increasing concentrations (10 to 40 μg/ml) of PGL (a) and PGPC (b). *Represents P < 0.001 as compared to control (untreated cells). NS = insignificant differences. The p values were obtained by one-way ANOVA, followed by Fisher’s LSD testBack to article page