Fig. 6From: Isolation of a novel bio-peptide from walnut residual protein inducing apoptosis and autophagy on cancer cellsInduction of autophagic cell death by CTLEW in MCF-7 cells. a Western blotting of LC3I/II. Cells were treated with (0.1, 0.5, and 1.0 mg/ml) or without (control) CTLEW for 48 h, and expression of LC3I/II in MCF-7 cells was detected by Western blotting. GAPDH was used as internal control and RAPA was used as a positive control. b Immunofluorescence for LC3-II. Cells were treated with (1.0 mg/ml) or without (control) CTLEW for 48 h, and formation of autophagic vacuoles was detected by immunofluorescent staining for LC3-II. RAPA was used as a positive control. Blue, DAPI-stained cell nuclei; Green, LC3-II-stained cytoplasm (100x magnification). Fluorescence intensity represents the expression of LC3-II. Data were presented as mean ± S.D. of three separate experiments. *p < 0.05; **p < 0.01; ***p < 0.001, significantly different from the respective control groupBack to article page