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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Isolation of a novel bio-peptide from walnut residual protein inducing apoptosis and autophagy on cancer cells

Fig. 3

Isolation of bioactive peptides with cytotoxic activity on MCF-7 cells. a IC50 value of WRPH-pa with different molecular weights on MCF-7 cell proliferation. b Gel filtration chromatography of WRPH-pa with <3 kDa molecular weights on a HiPrep 16/60 Sephacryl S-100HR column. Insets show the IC50 value of fraction I to V on MCF-7 cell proliferation. c RP-HPLC chromatography of fraction V from gel chromatography. Insets show the IC50 value of fraction A to D on MCF-7 cell proliferation. d, e Identification of fraction C by TOF-LC/MS/MS coupled with ESI source. d Amino acid sequence analysis of the bioactive peptide. e Molecular mass analysis of the bioactive peptide. Results were expressed as means ± S.D. of four separate experiments

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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