Fig. 1

Effects of walnut residual protein and the enzyme protein hydrolysates on MCF-7 cell proliferation. After treated with test substances (0.5, 1, 2, 3, 4 mg/ml) for 48 h, cell viability was measured by MTT assay. WRP, walnut residual protein; WRPH-pa, walnut residual protein hydrolysate prepared with papain; WRPH-pe, walnut residual protein hydrolysate prepared with pepsin; WRPH-tr, walnut residual protein hydrolysate prepared with trypsin; WRPH-np, walnut residual protein hydrolysate prepared with neutral protease; WRPH-ap, walnut residual protein hydrolysate prepared with alkaline protease. Results were expressed as means ± S.D. of four separate experiments. Statistical significance test for comparison with control (untreated) group was done by Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001